FastQCFastQC Report
Tue 4 Jul 2023
SRR5766481_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766481_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16375742
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length38-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG194614111.884292021698926No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT13090767.993995020195116No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT9180495.606152075429621No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT5881193.5914036750212603Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG4197372.563163244755566No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2729671.6668985136673502No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC2503281.5286513429437274No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC1562350.9540636387651932No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1242800.7589274427992332No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG1197690.7313805994256626No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1180310.7207673398860338No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1171730.7155278826449513No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1119440.6835965051232488No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC945990.5776776405001984No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT878690.5365802661033618No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA845930.5165750657283193No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG845690.5164285074838136No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT793870.48478413985760155No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG786860.48050341779932776No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT768950.46956650880308204No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC745680.4553564656795399No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA588340.35927532321894173No Hit
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT575570.3514772032925287No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG558910.34130361848641727Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT489140.29869791548987523No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG474220.2895868779564309No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC473110.28890904607559154No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG459600.28065903822861893No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT433980.2650139456276241No Hit
GCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT416290.25421138168884194No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT410670.2507794761299976No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG406970.24852003652719978No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT386470.2360015198089955No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA313920.19169818381359452No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG285160.1741356208469821No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCA278050.16979383285349756No Hit
TCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG263410.1608537799386434No Hit
TCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT250690.15308619297983567No Hit
ACTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT247330.15103437755675436No Hit
CCCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCA235270.1436698257703376No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA230260.1406104224162789No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA205380.12541721773584366No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCCAAG205290.125362258394154No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT200610.12250437262629077Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGTCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG198950.12149067810179229No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG188740.11525584611677443No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA179310.10949732842640046No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC177340.10829432950274864No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG175090.1069203459605067No Hit
ACATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG174560.10659669650388971No Hit
CCTTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGA172060.1050700481236209RNA PCR Primer, Index 1 (100% over 22bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA167890.10252359862533252RNA PCR Primer, Index 1 (100% over 22bp)
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT167320.10217552279463123No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG166860.10189461949266178No Hit
CCATTCCAGGGGACATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG165030.100777112878305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)