FastQCFastQC Report
Tue 4 Jul 2023
SRR5766485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16862484
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length30-70
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT364398021.609983440162196Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG7647374.535138476632506No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT7356684.362749877182982No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT3625822.1502288749391845No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2938771.7427859382994817Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1810371.0736081350766589No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1428540.8471705592129843No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT866140.5136491159902657Illumina Paired End PCR Primer 2 (96% over 27bp)
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT848330.5030872082665876Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC818580.4854444932312464No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG713220.4229625955464213No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT696540.4130708144778669No Hit
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT665560.3946986695435856Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA555870.32964894140151596RNA PCR Primer, Index 1 (100% over 22bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT542810.3219039377591107No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT539340.319846115198686No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC535580.3176163132311942No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA529120.3137853236806629Illumina Paired End PCR Primer 2 (100% over 26bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC518160.3072856881583994No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT498930.29588167437253016No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG490360.2907993863774756No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT479550.2843887057209365No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT466110.2764183497520294No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT462360.27419447810891917No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG419690.24888978397251557No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA418580.24823151796615492No Hit
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT356630.21149315842197391Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG350930.20811287352444632No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT343060.20344570823610567Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG301710.17892381691807685No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA290000.17197940706719128RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT279200.16557465673503383Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA266800.15822105450181598No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT256980.15239747595905798Illumina Paired End PCR Primer 2 (96% over 27bp)
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT254070.15067175156400445No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT249550.1479912449435089Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG246830.1463781967117063No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC244300.1448778246431546No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA236490.14024624130110366No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG229980.13638560012866432Illumina Paired End PCR Primer 2 (100% over 28bp)
CCAGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT223050.1322758853321966Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA222400.13189041424739084No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA221070.1311016811046344No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT212470.12600160213643494No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT210720.12496379536965016Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT195480.11592598101205019No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG188620.11185777848625386No Hit
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG182430.10818690769402656No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG179410.10639594973077514No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA175920.1043262665215872No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC171500.10170506314490795No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)