FastQCFastQC Report
Mon 3 Jul 2023
SRR5766488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16829781
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT445573126.475276178578916Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT14453898.588281689464646No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG4110892.442628338419852No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2457611.460274497927216No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT2072621.2315192930912173No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG1613310.9586042741732647No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1076450.6396102242803992No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT934400.5552062739259649Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT923750.5488782058423696No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT920030.5466678383990855No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG864490.5136668147969364No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG784490.46613203107039836Illumina Small RNA Adapter 2 (100% over 21bp)
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA663410.39418813590028295Illumina Paired End PCR Primer 2 (100% over 26bp)
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT661060.3927918016283159Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG619130.36787763310764415No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT518530.3081026425715225No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG416860.24769187430305836No Hit
CCATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAGAT401400.2385057773479049RNA PCR Primer, Index 1 (95% over 22bp)
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG388480.230828909776069Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAGAGCGGTTCAGCAGGAATG373620.22199932369886452Illumina Paired End PCR Primer 2 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA370250.21999692093438414No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC368440.2189214464525712No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA361240.21464331591718278No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT355800.21141095062377815Illumina Paired End PCR Primer 2 (100% over 27bp)
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT323240.19206429364707717Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC296980.17646100088884104No Hit
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT277950.16515366420989078No Hit
CCTGGAATTCTCGGGTGCCAAGAGATCGGAAGAGCGGTTCAGCAGGAATG275300.16357907449894923Illumina Paired End PCR Primer 2 (100% over 28bp)
CATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT273210.16233722827409341Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT271750.16146971847108407No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT269990.16042395322910025No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT268140.15932471135542406Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT266190.1581660510020897No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG264100.15692420477723387No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT257010.15271143456946945Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT254770.15138046062512636Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGA250980.14912850024608162RNA PCR Primer, Index 1 (100% over 22bp)
CCAGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT243190.14449980068071Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA242750.144238359370214No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA226340.1344877868583079RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA224510.13340042868056334No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC223060.13253886072551985No Hit
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT221150.13140396776404875No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT219390.1303582025220649Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAGGAGATCGGAAGAGCGGTTCAGCAGGAATG212070.12600876981108666Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA200890.11936578378530296No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGAGGTTCAGCAGGAAT196340.11666224296085612Illumina Paired End PCR Primer 2 (96% over 27bp)
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG190690.11330509886016936No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC188230.11184340426057832No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG187670.11151066077449256No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG180480.10723847208706994No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)