FastQCFastQC Report
Tue 4 Jul 2023
SRR5766489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17696910
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length34-70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT506797928.637649171522035Illumina Paired End PCR Primer 2 (100% over 27bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG9244965.22405323867274No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT4620282.6107834644579198No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT4393142.482433373961895No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1939171.0957675662022353No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA1138520.6433439510061362Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT1002790.5666469457097313Illumina Paired End PCR Primer 2 (96% over 27bp)
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT896900.50681164112831Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC798560.45124261806157123No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG778210.43974343543590383No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC705300.39854415262325454No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG697010.3938597190130932Illumina Small RNA Adapter 2 (100% over 21bp)
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT617830.34911744479686Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT591670.3343352031512846No Hit
CCTGGAATTCTCGGGTGCCAAGAGATCGGAAGAGCGGTTCAGCAGGAATG569120.32159286564716666Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC561960.31754696158820944No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT553800.31293598712995657No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT535910.3028268776865566No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG491780.2778903209656375No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG481290.2719627324770256Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAGAGCGGTTCAGCAGGAATG480340.27142591559769474Illumina Paired End PCR Primer 2 (95% over 22bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT435120.24587343214154336No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG409260.23126071161575665No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA390780.22081821063677218No Hit
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT386860.21860313467153306Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT373490.2110481434329496Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAATCGGAAGAGCGGTTCAGCAGGAATG361650.20435770990528856Illumina Paired End Adapter 2 (96% over 27bp)
CCTGGAATTCTCGGGTGCCAGGAGATCGGAAGAGCGGTTCAGCAGGAATG354390.2002552988064018Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT352490.19918166504774No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG344230.19451418354955752No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT328670.1857216881365165No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT318820.18015574470345389No Hit
CCAGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT311060.17577079840491927Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT309100.1746632604222997Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT304060.1718153056098494No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG299080.16900125502135682No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT270600.1529080500494154Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG269150.15208869797043664No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT266820.15077208394007766Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT262400.1482744727751907No Hit
GCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG259810.14681094044101484No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCTGGAATTCTCGGGTGCCAA255520.14438678842803632No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC255130.14416641097231098No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG235980.1333453128257984No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT229580.12972886227030594Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC208560.1178510824771104No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG206610.11674919519848381No Hit
CATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT193670.1094371842315975Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGAGGTTCAGCAGGAAT193070.10909814199202009Illumina Paired End PCR Primer 2 (96% over 27bp)
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG188990.10679265476289364Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG182990.10340223236711946Illumina Paired End PCR Primer 2 (100% over 28bp)
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT182430.10308579294351387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)