FastQCFastQC Report
Tue 4 Jul 2023
SRR5766490_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766490_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16610532
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length32-70
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT651191239.20351256660533Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT6118953.683777256502079No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG2363091.4226455841390269No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2009691.2098890029530662No Hit
GCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT1873771.128061401043627Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT1288920.7759655139281512Illumina Paired End PCR Primer 2 (96% over 27bp)
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA1104860.6651562996296567Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT1091670.6572155545650193No Hit
CCTGGAATTCTCGGGTGCCAAGAGATCGGAAGAGCGGTTCAGCAGGAATG824210.4961972319730638Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA784350.47220040875271185No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC602390.36265545257671455No Hit
CCTGGAATTCTCGGGTGCCAGGAGATCGGAAGAGCGGTTCAGCAGGAATG599790.36109018061552756Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG596120.3588807390395443Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT567780.3418192746626056No Hit
CCGAAAAGAAACCAACCGGGATTGCCTTAGTAACGTGGAATTCTCGGGTG564620.339916867202086No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC549090.33056737737238034No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG532770.32074228567754487No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT487330.2933861480174145No Hit
CCTGGAATTCTCGGGTGCCAAGGAATCGGAAGAGCGGTTCAGCAGGAATG487160.29328380331225995Illumina Paired End Adapter 2 (96% over 27bp)
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT482800.29065896263888474Illumina Paired End PCR Primer 2 (100% over 27bp)
ACTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT467800.2816285474781903Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTTGGAATTCTCGGGT452650.2725078281658889No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAGAGCGGTTCAGCAGGAATG448270.2698709469389662Illumina Paired End PCR Primer 2 (95% over 22bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC434750.26173153274079364No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT412830.24853508605263216No Hit
CCAGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT412820.24852906577585834Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG403810.24310479640266788Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT383100.2306368032041358Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA358670.21592926704575147No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT358150.21561621265351405Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT350050.21073978846673905Illumina Paired End PCR Primer 2 (96% over 27bp)
TCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT330510.19897616765074108Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC323210.19458136560586983No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT311520.18754366205730194Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTGACCTCAAATCAGGTAGGAGTACCCGCTGAATGGAATTCTCGGGTG304620.18338967108338253No Hit
CCTGGAATTCTCGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG281870.16969354142299597Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG281520.1694828317359131No Hit
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG266250.16028986910232615Illumina Paired End PCR Primer 2 (100% over 28bp)
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA260920.15708106158189275RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG254690.15333042915181763No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGGCGGTTCAGCAGGAATG247660.14909817457983887RNA PCR Primer, Index 1 (95% over 22bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGAGGTTCAGCAGGAAT243420.14654557722774925Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATTGGAATTCTCGGGTGCCAA242100.14575090069360813No Hit
CCTTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTGGAATTCTCGGG239650.14427593288402804No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC235860.14199424798675925No Hit
CCTGGATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG233470.14055540183782195Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG232840.14017612440107277Illumina Paired End PCR Primer 2 (100% over 28bp)
CCACGACGCTCTTCCGATCTTTCCGCCTGGAATTCTCGGGTGCCAAGGAG224010.13486022000981066RNA PCR Primer, Index 1 (95% over 22bp)
CCTCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGT213200.12835230081733687No Hit
CCATTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT195820.1178890597844789No Hit
CCACGCTCTTCCGATCTTTCCGCCTGGAATTCTCGGGTGCCAAGGAGATC194760.1172509104464565RNA PCR Primer, Index 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATATGGAATTCTCGGG193960.11676928830455278No Hit
CATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT193860.11670908553681485Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAAGCGGTTCAGCAGGAATG193330.11639001086780362RNA PCR Primer, Index 1 (95% over 22bp)
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA192900.11613113896653039No Hit
CCATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTGCCAAGGAGAT192290.11576390208332882RNA PCR Primer, Index 1 (95% over 22bp)
GCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT184430.11103196453912494No Hit
CCTGGAATTCTCGGGTGCCAAGGAGTCGGAAGAGCGGTTCAGCAGGAATG183290.11034565298691215Illumina Paired End PCR Primer 2 (96% over 26bp)
CCACACGACGCTCTTCCGATCTTTCCGCCTGGAATTCTCGGGTGCCAAGG181050.10899711098958179Illumina Single End PCR Primer 1 (95% over 22bp)
CCTTTGCTCGAATATATTAGCATGGAATTGGAATTCTCGGGTGCCAAGGA179630.1081422316877027RNA PCR Primer, Index 1 (100% over 22bp)
CCCACGACGCTCTTCCGATCTTTCCGCCTGGAATTCTCGGGTGCCAAGGA178040.1071850076806691RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCA177660.10695623716326486No Hit
CCTGGAATTCTCGGGTGCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG174200.10487322139953134Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGTGAAGCGGCAAAAGCTCAAATTTGAAATCTGGTGGAATTCTCGGGTG166460.10021352717661301No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)