FastQCFastQC Report
Tue 4 Jul 2023
SRR5766492_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766492_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23943754
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length48-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT310690612.97585165634428Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT7060572.9488149602606173No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG5203882.173376823032846No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG2302830.9617664798928356Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT1252210.522979813441117No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA1111770.4643256859388048Illumina Paired End PCR Primer 2 (100% over 26bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT929730.3882975075671092No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT912200.38097618276565987No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA892300.3726650382392001No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA712810.29770185577416136No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA684040.2856861960743499No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG681880.2847840818945935No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG677530.28296732417147286No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA664330.2774544041840724No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA649490.2712565456527828No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA640120.26734320775263565No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT601630.25126803424392014Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA516180.21558023023457393No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC503910.210455720519013No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG477600.19946746863503526No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG459980.1921085557427628No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC396790.165717539530351No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA387490.16183343681195522No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG385600.16104408690466832No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA362770.15150924119918704RNA PCR Primer, Index 1 (100% over 22bp)
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC358200.14960060147627643No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGT349180.1458334394848861No Hit
CCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTG343980.1436616831262132No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCC338040.141180869131883No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT334580.13973581586245834Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG331740.13854970277426004No Hit
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG325730.13603965359817846Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA311870.13025108761140797No Hit
CCTTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCC296020.1236314071719915No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT289990.1211130050868381No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG286400.11961365790844661No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA279220.11661496355166363RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT278980.11651472864280178Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGGCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGG270100.11280603701491419No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC268390.1120918632892737No Hit
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG268210.11201668710762731No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCA263170.10991175402152896No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG257150.10739752839091148Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAG252910.10562671166768585No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG252390.10540953603181857No Hit
CCGGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA249990.10440718694320031No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)