FastQCFastQC Report
Tue 4 Jul 2023
SRR5766493_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766493_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20903058
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length45-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT362043617.320126079160282Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG4694542.245862782373756No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG3068221.4678330797340753Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1747580.83604035352148No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA728680.3485997120612688No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA725530.34709275551931207Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT683280.32688040190100415Illumina Paired End PCR Primer 2 (96% over 27bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA663810.31756597527500524No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA658150.3148582374885053No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC646870.3094618978715937No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC606210.2900101985077973No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT603050.2884984579768185No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA570060.27271607819296106No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG568440.27194107197138334No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA548970.2626266453453844RNA PCR Primer, Index 1 (100% over 22bp)
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA532340.2546708716016575No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA530970.25401546510563194No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA486800.23288458559508374No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG483860.23147809282259083No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA443430.21213642520630233No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG432360.20684054935885457No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA408890.19561252712402177No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC393460.18823083206294505No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC372350.1781318312373242No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA361030.17271635566432433RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT347800.16638713818810624Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAG318890.15255662592525934No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC299990.14351488667351925No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT296980.14207490597787176Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG277680.13284180716524827No Hit
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG277380.13269828749458573No Hit
CCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTG271800.13002882162026244No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT249470.1193461741339473No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG247040.11818366480158071Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG235870.11283994906391209No Hit
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA231710.11084980963072485No Hit
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG230460.11025181100296426No Hit
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG228760.10943853286920985Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCA222860.10661597934617988No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT221050.10575007733318255Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG217140.10387953762554741No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGT216360.10350638648182482No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC215740.10320977916245556No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)