FastQCFastQC Report
Tue 4 Jul 2023
SRR5766496_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766496_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20828371
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length45-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT285541713.709267037734252Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT6028252.8942493870500003No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG5829862.7989994992887346Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2944421.4136583221030583No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA1628280.7817606091230082RNA PCR Primer, Index 1 (100% over 22bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC1364340.6550392251031057No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG1038280.49849313707730675No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1038190.4984499267849608No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG1017850.4886844007147751No Hit
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG903320.43369690313275094No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT792430.38045702181894114No Hit
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA778240.37364419905906227Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG692990.3327144499202554No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT672190.32272807124474595Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG625700.30040755467626346Illumina Small RNA Adapter 2 (100% over 21bp)
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG605580.2907476537651456No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC560090.26890725155606265No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA557320.26757733478052603RNA PCR Primer, Index 1 (100% over 22bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT538060.2583303322184918No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG535590.25714444975077505No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC488500.23453586456665285No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG467130.22427582070628566No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA456480.21916260277868105No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG451400.21672362183293165No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT448720.215436915349741No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA440960.2117112279208009No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC431800.20731338038870156No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG399360.19173847056978194No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG384990.18483922722521123Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG384270.1844935448864436No Hit
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG383320.18403743624501406Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT370930.17808881933205434Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT365570.17551540636567306No Hit
CCTAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG355010.1704453987304144No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC340900.16367098511928754No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG321210.1542175333827115No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA314800.15114000033896074No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT310720.1491811337526108No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT302830.1453930314569488Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG297250.14271399333149962Illumina Paired End PCR Primer 2 (100% over 28bp)
CCCCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG284070.1363860860746143No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA262080.1258283713114194No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG259710.12469050027964261No Hit
CTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG255900.12286126457033053Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA245800.11801210954039565No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG236060.11333579567984459No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCAAAGG234340.11250999898167743Illumina Small RNA Adapter 2 (95% over 21bp)
CCCCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG233050.1118906514580521Illumina Small RNA Adapter 2 (100% over 21bp)
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG229700.1102822683540638No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT227250.10910598817353502Illumina Paired End PCR Primer 2 (96% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA227060.10901476644524914No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT218330.10482336808769153Illumina Paired End PCR Primer 2 (96% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)