FastQCFastQC Report
Tue 4 Jul 2023
SRR5766498_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766498_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18055107
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length46-70
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT328581418.198806575890135Illumina Paired End PCR Primer 2 (100% over 27bp)
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG6406513.548309073992195Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT3456541.9144389451693637No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2044671.13246074919412No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA1210160.6702591128371601RNA PCR Primer, Index 1 (100% over 22bp)
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG1149810.6368336670616241Illumina Paired End PCR Primer 2 (100% over 28bp)
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA1001860.5548900928695687Illumina Paired End PCR Primer 2 (100% over 26bp)
CCTTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG988920.5477231455897769No Hit
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG836480.4632927403864181No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT811190.44928562317575854Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT585130.3240800511456398No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA565790.3133684004198923No Hit
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG515430.28547601517952786No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG462680.25625990474606436No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT438800.24303373001334194Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT436090.24153276964794507No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG434230.24050259020896417No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA409610.22686655914030307RNA PCR Primer, Index 1 (100% over 22bp)
CCTCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG384830.2131419104854931Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA381880.21150802374087285No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT377080.20884949615640602No Hit
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT359000.198835708921581Illumina Paired End PCR Primer 2 (100% over 27bp)
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA354470.19632672351374048No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG330850.18324455235850998No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT328050.18169374460090432No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA307710.17042823396172618No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA301530.16700537969672513No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATTGGAAGAGCGGTTCAGCAGGAAT292740.1621369510576703Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG289770.16049198711478144Illumina Small RNA Adapter 2 (100% over 21bp)
CCTAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG280160.15516939334671348No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT270710.14993541716479442Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA270410.14976925919076525No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC269080.14903262550590257No Hit
CCTGGAATTATCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT266170.14742089315781956Illumina Paired End PCR Primer 2 (100% over 27bp)
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA265150.14685595604612037No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC264660.14658456468853936No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC254330.1408631917828014No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT252170.13966685436979132No Hit
CCCCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG251700.13940654021047894No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCAAAGG251190.13912407165462937Illumina Small RNA Adapter 2 (95% over 21bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG241110.13354116372724903No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACTGGAATTCTCGGGTGCCAAG233660.12941490737219113No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG230640.12774225043363077Illumina Paired End PCR Primer 2 (100% over 28bp)
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG223330.12369353446645318No Hit
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA220640.1222036512993249No Hit
CCAAGAGGTGCACAATCGACCGATCCTGGAATTCTCGGGTGCCAAGGAGA216830.12009344502915435RNA PCR Primer, Index 1 (95% over 22bp)
CCCCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG212960.11795000716417797Illumina Small RNA Adapter 2 (100% over 21bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG208800.11564594992430674No Hit
CCATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAAGG200000.11077198268611756Illumina Small RNA Adapter 2 (100% over 21bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG198310.10983595943241987No Hit
CCTGGAATTCTTGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT197630.10945933469128706Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG193130.10696696508084942No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG188440.10436936208685996No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)