FastQCFastQC Report
Tue 4 Jul 2023
SRR5766502_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766502_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10988600
Total Bases746.7 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT177588916.16119432866789Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT1902331.7311850463207323No Hit
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG1455121.3242087254063302No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG1029400.9367890359099431Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC585600.5329159310558215No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC535290.4871321187412409No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT502050.4568825874087691No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG431590.3927615892834392No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA379390.3452578126421928No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT348430.3170831589101432Illumina Paired End PCR Primer 2 (96% over 27bp)
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA331440.30162168065085637Illumina Paired End PCR Primer 2 (100% over 26bp)
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT304800.27737837395118575No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA298550.27169066123073005No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC298240.2714085506797954No Hit
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT293660.2672405947982454No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA285500.2598147170704184No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA285400.2597237136668912No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA283340.25784904355422894No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGTGGAATTCTCGGGTGCCAA282310.25691170849789785No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT266970.24295178639681123No Hit
CCTAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCA253390.230593524197805No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG250420.22789072311304442No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC248940.2265438727408405No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT231860.21100049141837904No Hit
CCAAATATAGATGGATACGAATAAGGCGTCCTGGAATTCTCGGGTGCCAA201710.18356296525490054No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG200390.18236172032834028No Hit
CCTTGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGC193250.17586407731649162No Hit
CCTGGAATTCTCGGGTGTCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT185100.16844729992901733Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG181610.16527128114591486No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG181360.16504377263709663No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG176730.160830315053783No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT171860.1563984493020039No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTATGGAATTCTCGGGTGCC170490.15515170267368No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG169390.15415066523487977No Hit
CCGGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA164070.14930928416722786No Hit
CCGTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATT158380.14413119050652493No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA156760.14265693536938281RNA PCR Primer, Index 1 (100% over 22bp)
CCTGGAATTCTCGGGTACCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT152780.1390349999089966Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTGTACAACGGGGTATTGTAAGCAGTAGAGTTGGAATTCTCGGGTGCCA148210.13487614436779935No Hit
CCTGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG138320.1258759077589502Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA134380.12229037365997489No Hit
CCTTGAGATTAAGCCTTTGTTGTCTGATTTGTTGGAATTCTCGGGTGCCA126090.11474619150756238No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGTAGGAAT125250.11398176291793312Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC122750.11170667782975084No Hit
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG120440.1096044992082704Illumina Paired End PCR Primer 2 (100% over 28bp)
CCTTGTGGCGTCGCTGAACCATAGCAGGCTAGCATGGAATTCTCGGGTGC116710.10621007225670241No Hit
CCTCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAA114550.10424439874051289No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG112760.10261543781737437No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG109910.10002184081684656No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)