FastQCFastQC Report
Tue 4 Jul 2023
SRR5766506_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766506_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13216534
Total Bases890.3 Mbp
Sequences flagged as poor quality0
Sequence length30-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAAT9820847.430722759840061Illumina Paired End PCR Primer 2 (100% over 27bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT5873784.444266552789105No Hit
CCAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG4193503.172919617200697Illumina Small RNA Adapter 2 (100% over 21bp)
CCTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG2936902.222140842674789No Hit
CCAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGGA1315680.9954803581634943RNA PCR Primer, Index 1 (100% over 22bp)
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC1012930.7664112240016937No Hit
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT757620.5732365232821253No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG753060.5697862994942547No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGT730560.5527621689619987No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG633810.4795584076732977No Hit
CCAAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAAG588150.4450107721131728No Hit
CCTTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG579670.43859456647257145No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGT462460.34991019581987226No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG411100.31104978052490917No Hit
CCTAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAGG391910.29653008875095394Illumina Small RNA Adapter 2 (100% over 21bp)
CCCTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG390590.29553133975972823No Hit
CCAAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG382030.28905460387723436No Hit
CCGTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGTG376450.28483261950523486No Hit
CCCCCCACTAATAGGGAACGTGAGCTGGGTTTTGGAATTCTCGGGTGCCA360990.27313515025951585No Hit
CCCTTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCGTTGGAATTCT357870.2707744708257097No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGC354470.2682019355452799No Hit
CCTTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGC347450.262890406819216No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTGCC307000.2322848032623379No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGG304350.23027973899965No Hit
CCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG300400.22729105830620946No Hit
CCTGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA299740.2267916838105966No Hit
CCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCC298470.22583076622055376No Hit
CCTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTGCCA293200.22184333653588756No Hit
CCAAGAGGTGCACAATCGACCGATCCTGTGGAATTCTCGGGTGCCAAGGA266460.20161110318333084RNA PCR Primer, Index 1 (100% over 22bp)
CCATGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAA261680.19799442123025596Illumina Paired End PCR Primer 2 (100% over 26bp)
CCGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGCGGTTCAGCAGGAATG233760.17686936680978538Illumina Paired End PCR Primer 2 (100% over 28bp)
CCCCTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG233730.1768466679690757No Hit
CCGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAAG222450.16831190386223802No Hit
CCTAAGAGGTGCACAATCGACCGATCCTGATGGAATTCTCGGGTGCCAAG217780.16477845099176533No Hit
CCTATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGT205480.1554719263007987No Hit
CCTTGTGAAACTCCGTCGTGCTGGGGATAGAGCATTGGAATTCTCGGGTG203960.15432185170484183No Hit
CCTGGAATTCTCGGGTGCCAAGGAGATCGGAAGAGTGGTTCAGCAGGAAT197060.14910111834161663Illumina Paired End PCR Primer 2 (96% over 27bp)
CCTTTGCTCCACACAATATAGATGGATACGAATAAGGCGTCTGGAATTCT195260.14773918789903617No Hit
CCGATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG177010.13393072646731738No Hit
CCTCTATGCCGACTAGGGATCGGGTGGTGTGGAATTCTCGGGTGCCAAGG171810.1299962607443071Illumina Small RNA Adapter 2 (100% over 21bp)
CCTCAATATAGATGGATACGAATAAGGCGTCTGGAATTCTCGGGTGCCAA169510.1282560162898987No Hit
CCCTTCCAGGGGGCATGCCTGTTTGAGCGTCATTTTGGAATTCTCGGGTG164050.1241248272807379No Hit
CCCATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTGGAATTCTCGGGTG163700.12386000747245836No Hit
CCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGGGTG162150.12268723403579185No Hit
CCAGTTTCTTTACTTATTCAATGAAGCGGAGTGGAATTCTCGGGTGCCAA161880.12248294446940476No Hit
CCTTTTGCTCGAATATATTAGCATGGAATAATAGAATGGAATTCTCGGGT155340.11753459719469567No Hit
CCTTTTGATGATTCATAATAACTTTTCGAATCGCATGGAATTCTCGGGTG147820.11184475445680388No Hit
CCTTTGATGATTCATAATAACTTTTCGAATCGCATTGGAATTCTCGGGTG147260.1114210427635566No Hit
CCGCCAAGGACGTTTTCATTAATCAAGAACGATGGAATTCTCGGGTGCCA142660.10794055385473984No Hit
CCGGCTTGCTCGAATATATTAGCATGGAATAATAGAATTGGAATTCTCGG136700.10343105083375113No Hit
CCACTATGCCGACTAGGGATCGGGTGGTGTTGGAATTCTCGGGTGCCAAG135700.10267442281009528No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)