FastQCFastQC Report
Mon 3 Jul 2023
SRR5766576_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766576_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27812481
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18352966.598821586610702No Hit
GTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGT1003460.36079485321715815No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC993720.35729282835285353No Hit
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCC852850.3066429061111089No Hit
TCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTG718210.2582329853996125No Hit
GTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC604410.21731610351482128No Hit
CCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGG592960.21319924676982252No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC574020.20638935447722193No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC565110.2031857567830788No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG517910.18621495867269086No Hit
CGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAG506800.18222034920221608No Hit
TGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCC495430.1781322565218112No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG487610.17532056920775965No Hit
CGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCT443790.15956505282646305No Hit
AATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTG387460.1393115558442988No Hit
AATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGC386480.13895919605302381No Hit
CATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATG352760.12683514282670433No Hit
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT343640.12355603946300224No Hit
ATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCA326830.11751199039021366No Hit
TGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCG322600.11599109047481237No Hit
TCAAATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCACGTCAACAG315160.11331603246758173No Hit
ATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTC303100.10897984973005466No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG302040.10859872587418576No Hit
CCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTA296980.10677939878862298No Hit
ATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGC293310.10545984732537884No Hit
TCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA288930.10388501478886404No Hit
AATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATT287920.10352186847336634No Hit
GAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTAT285140.10252231722872908No Hit
GTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTT284630.1023389463169431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)