FastQCFastQC Report
Tue 4 Jul 2023
SRR5766577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35888607
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN32491669.053474825590193No Hit
GTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGT828440.2308364880252945No Hit
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCC621890.1732834043962754No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC574140.15997834633146948No Hit
TCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTG528410.1472361409847978No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC472090.13154313846731358No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC463950.12927500919720844No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG443850.12367434601181372No Hit
GTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC442840.12339291965274664No Hit
CCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGG416090.11593930073686058No Hit
CGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAG406020.11313339634497377No Hit
CGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCT377270.10512249751014298No Hit
TGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCC376380.10487450794621257No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG373600.10409988885887937No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)