FastQCFastQC Report
Tue 4 Jul 2023
SRR5766578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21447997
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12560845.85641633575387No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC859440.40070874683542707No Hit
GTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGT783230.365176291287247No Hit
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCC754710.35187901229191704No Hit
GGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGT669840.3123088836687174No Hit
TCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTG537520.2506154770536382No Hit
GTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC524120.24436780739945088No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG473070.2205660509930135No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC458430.21374023877381185No Hit
CCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGG433200.20197690255178607No Hit
TGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCC415700.1938176324810191No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC409620.19098286893643263No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG399840.186423002576884No Hit
CGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAG385040.17952259131703535No Hit
CGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCT315820.14724918135712162No Hit
AATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTG304910.14216245927300344No Hit
AATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGC295980.13799890031689208No Hit
ATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTC278220.12971840680507368No Hit
TGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCG268110.1250046799241906No Hit
GAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTAT266850.12441721247909536No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG258990.12075253460731089No Hit
AATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATT258870.12069658532682563No Hit
ATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCA256500.11959158703724175No Hit
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT252880.1179037837426031No Hit
TCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA252500.11772661102106644No Hit
CATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATG249570.11636051608921803No Hit
TCAAATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCACGTCAACAG246810.11507368263805706No Hit
CCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTA242340.11298957193998116No Hit
TTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCC239000.11143231696647476No Hit
ATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGC236480.11025738207628433No Hit
GTGAAACTCCGTCGTGCTGGGGATAGAGCATTGTAATTATTGCTCTTCAA228480.1065274300439337No Hit
GTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTT221800.10341292009692094No Hit
AATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCC220680.10289072681239186No Hit
ATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCT217910.10159923092119044No Hit
GCGAAATGCGATACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAAT214750.10012589986841197No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)