FastQCFastQC Report
Thu 6 Jul 2023
SRR5766582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29335237
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24458488.33757709201395No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC1189690.40554981710221055No Hit
GTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGT1008030.3436242904736035No Hit
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCC929720.31692943200015733No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC707880.24130706699250462No Hit
TCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTG704190.24004919408014327No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC689440.23502111130037912No Hit
GTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC666620.22724207068788976No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG601040.20488670331860623No Hit
CCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGG591520.2016414593821076No Hit
CGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCT559280.19065126352993159No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG542530.18494140681392823No Hit
TGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCC536810.1829915333562841No Hit
CGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAG508760.17342965390052925No Hit
AATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGC452860.15437407238264345No Hit
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT427460.14571554339240553No Hit
CATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATG424950.14485991710242532No Hit
AATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTG422660.14407928594543143No Hit
ATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCA390120.13298682400281953No Hit
TGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCG357580.12189436206020765No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG355200.12108305107608301No Hit
ATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTC354730.1209228342010668No Hit
TCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA340720.11614700777771116No Hit
AATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATT333670.11374375465246794No Hit
ATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGC325370.11091439281707524No Hit
GAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTAT323770.11036897366808389No Hit
TTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCC318540.10858613482481835No Hit
CCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTA299940.10224563721779376No Hit
TCAAATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCACGTCAACAG298470.10174453337465793No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA297970.10157408989059812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)