FastQCFastQC Report
Thu 6 Jul 2023
SRR5766584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25062856
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12598685.026833334556924No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC1378760.5501208641185984No Hit
GTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGT995480.3971933605651327No Hit
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCC912000.36388510551231673No Hit
GGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGT848350.3384889575234363No Hit
TCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTG671220.2678146496951505No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG645400.25751255164215925No Hit
GTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC615430.24555461676035645No Hit
CCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGG595860.237746248871238No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC543510.21685876501863952No Hit
TGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCC503750.20099465120814644No Hit
CGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAG498040.19871637933043226No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG470290.1876442174028371No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC452060.18037050526085296No Hit
ATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTC375890.14997891700770255No Hit
AATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATT373180.14889763560864733No Hit
TCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA360440.14381441604260903No Hit
AATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTG358700.14312016156498686No Hit
GAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTAT349690.13952520016074785No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG346850.13839204917428405No Hit
CGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCT346270.13816063101507664No Hit
TTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCC342340.13659257348803347No Hit
AATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGC340210.1357427102481856No Hit
CCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTA336800.13438213107077662No Hit
TGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCG324290.12939068077476884No Hit
AATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCC322970.12886400496415892No Hit
ATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCT312470.12467453828885262No Hit
ATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCA299930.11967111808805829No Hit
TTGACCTCAAATCAGGTAGGAGTACCCGCTGAACTTAAGCATATCAATAA290980.11610009649339245No Hit
CATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATG285090.11375000518695874No Hit
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT281130.11216997775512895No Hit
ATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGC278760.11122435527698839No Hit
TTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTT267870.10687927983945644No Hit
GTTTCCCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTA254290.10146090293939367No Hit
GTATACGACTTAGATGTACAACGGGGTATTGTAAGCAGTAGAGTAGCCTT252080.10057911995344825No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)