FastQCFastQC Report
Tue 4 Jul 2023
SRR5766588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28466386
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20159327.081798160117692No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC1405660.49379643766511144No Hit
GTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGT1206050.42367513740592144No Hit
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCC1078320.37880467158704306No Hit
TCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTG844120.2965321976593727No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC780920.27433057361057356No Hit
GTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC773450.27170642595797023No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC749690.26335973944848495No Hit
CCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGG730380.2565763002019294No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG685060.24065576852642973No Hit
TGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCC612400.21513092670070588No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG609320.21404894881984665No Hit
CGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAG598570.21027256498243224No Hit
CGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCT596690.20961213692528444No Hit
AATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTG488650.17165860113047018No Hit
AATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGC482110.16936115459124315No Hit
CATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATG459770.16151330203981634No Hit
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT454330.1596022761723248No Hit
ATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCA427820.15028953798350095No Hit
TGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCG407240.14305995850685085No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG405830.14256463746398998No Hit
ATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTC400120.14055876288616337No Hit
TCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA390270.13709854141653247No Hit
AATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATT378690.1330305856177177No Hit
ATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGC366590.12877995822862795No Hit
TTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCC364750.12813358183227053No Hit
CCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTA363350.12764177370460725No Hit
GAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTAT362550.12736074048879967No Hit
AATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCC316570.11120835641025877No Hit
ACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCAC314280.11040389883000955No Hit
ATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCT301590.10594600944426173No Hit
TAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATT298040.10469892454911557No Hit
TCAAATGAGAACTTTGAAGACTGAAGTGGGGAAAGGTTCCACGTCAACAG289030.10153378795608266No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)