FastQCFastQC Report
Tue 4 Jul 2023
SRR5766590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25775594
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17082856.627529126971816No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC1350240.5238443777474148No Hit
GTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGT1142690.44332247008546144No Hit
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCC1024310.397395303479718No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC965300.37450155367903454No Hit
GGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGT938030.363921778097529No Hit
GTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC745870.2893706348726629No Hit
TCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTG738800.28662773009227255No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG705030.27352618915397253No Hit
CCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGG698330.27092683101696896No Hit
AATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTG568170.2204294496569119No Hit
CGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAG556770.2160066611849954No Hit
TGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCC552370.21429962002039604No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC543870.21100192686151092No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG541180.20995830396769907No Hit
CGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCT430890.1671697653214122No Hit
ATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTC393300.1525862022811191No Hit
AATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGC383760.14888502666514689No Hit
TCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCA382860.14853585915420608No Hit
CCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAG379420.14720126333461026No Hit
AATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATT376860.14620807574793426No Hit
TGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCG376270.14597917704631755No Hit
GAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTAT365850.14193659319742544No Hit
CCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTA361140.14010928322350205No Hit
TTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCC358600.13912385491484697No Hit
CATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATG356280.13822377866442187No Hit
ATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCA345390.13399885178203846No Hit
TCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCAT340900.13225689386634504No Hit
ATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGC329360.1277797904482822No Hit
ACGTAATGTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCAC325270.1261930180930069No Hit
AATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCC322780.1252269879794041No Hit
ATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCT319100.12379928082355736No Hit
CGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGGG301710.11705258858437947No Hit
GTTTCCCGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTA259460.10066111376521526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)