FastQCFastQC Report
Tue 4 Jul 2023
SRR5766593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5766593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29336697
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length35-70
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29087429.915028948214585No Hit
GTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGT747950.25495371888662177No Hit
GGTTGCGGCCATATCTACCAGAAAGCACCGTTTCCCGTCCGATCAACTGT724180.24685123891077446No Hit
GAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCCC625300.21314601299525981No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGCATCGATGAAGAACGCAGC573520.19549576423003584No Hit
TCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCC484730.16522991664671727No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGC474750.16182803401487222No Hit
GTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGG470570.16040319740153433No Hit
TCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTG462040.15749557627431607No Hit
GTGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGC457010.15578100015826593No Hit
CCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAGTGG412300.1405407023156015No Hit
CGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGCATGCCT378190.12891362650676044No Hit
CGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGG371590.12666388448570062No Hit
CGTCCGATCAACTGTAGTTAAGCTGGTAAGAGCCTGACCGAGTAGTGTAG355330.12112133823381685No Hit
TGAATTGCAGAATTCCGTGAATCATCGAATCTTTGAACGCACATTGCGCC353960.12065434632944534No Hit
AATCATCGAATCTTTGAACGCACATTGCGCCCCTTGGTATTCCAGGGGGC294150.10026691143859856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)