FastQCFastQC Report
Wed 27 Sept 2023
SRR578265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR578265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29340207
Total Bases1.4 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAGCTCGACAAAGAGAACGCCTTGGATCTGGAATTCTCGGGTGCCAA3350251.1418631095547485No Hit
AAGGGCACTGAAGATGAACTGGACAAATACTTGGAATTCTCGGGTGCCAA1702780.580357186982355No Hit
CTGAAGCTCGACAAAGAGAACGCCTTGGATTGGAATTCTCGGGTGCCAAG1084070.3694827374598959No Hit
AAGGGCACTGAAGATGAACTGGACAAATACTGGAATTCTCGGGTGCCAAG1029610.35092117789080357No Hit
CGAAAGTCAGAGAAGGAACGCCTAGAGGCCTGGAATTCTCGGGTGCCAAG823240.2805842508200436No Hit
CGGAACCCAAAAGAACCACTGATCAGCGGGTGGAATTCTCGGGTGCCAAG570380.1944021730998694No Hit
ATCATGATCACTGGAGACAACAAAGGCACCGTGGAATTCTCGGGTGCCAA563210.19195842755983283No Hit
CAAGCACGTCAGAATCTCCAGAACCTATTTTGGAATTCTCGGGTGCCAAG556050.18951809031204175No Hit
ATCATGATCACTGGAGACAACAAAGGCACCTGGAATTCTCGGGTGCCAAG515870.17582357206954946No Hit
CACCGGGAAAATCTGAACAAGCTGATGACATGGAATTCTCGGGTGCCAAG482040.16429331940296127No Hit
TGTGACGACGAGGAGACCACCGCTTTGGTGTTGGAATTCTCGGGTGCCAA443400.15112367816627878No Hit
CATCATGATCACTGGAGACAACAAAGGCACCTGGAATTCTCGGGTGCCAA409800.1396718162213375No Hit
TGTGACGACGAGGAGACCACCGCTTTGGTGTGGAATTCTCGGGTGCCAAG395800.13490020707761197No Hit
AAGGGCACTGAAGATGAACTGGACAAATATGGAATTCTCGGGTGCCAAGG395710.1348695324474023Illumina Small RNA Adapter 2 (100% over 21bp)
CCTGAGACCCTGGATAAGTTTGACAAGTTCTGGAATTCTCGGGTGCCAAG392470.13376524575985438No Hit
AAGATGATGGATTTTGAGACGTTCCTGCCTTGGAATTCTCGGGTGCCAAG372440.12693843639208136No Hit
CACCATGTACCCTGGTATTGCCGATCGTATGTGGAATTCTCGGGTGCCAA363940.12404138798339086No Hit
CACATCTCCAAGAACAAGGACACTGGCACGTGGAATTCTCGGGTGCCAAG320030.10907557673332025No Hit
GGAGAATCCGGAGCGGGGAAGACTGTGAACTGGAATTCTCGGGTGCCAAG309730.10556503572043646No Hit
GAAATCTGAAATCGGGATTGCCATGGGCTCTGGAATTCTCGGGTGCCAAG302400.1030667575044716No Hit
ACCATGTACCCTGGTATTGCCGATCGTATGTGGAATTCTCGGGTGCCAAG296050.10090249192856753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)