FastQCFastQC Report
Thu 28 Sept 2023
SRR5859396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5859396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44531109
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT10022612.250698494843234Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC8735531.961669088456791Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT8277431.8587971837844866Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC7226911.622890191214416Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT4490301.0083512629339637Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT4210310.9454761164829737Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG4137540.9291347314076548Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG3641370.8177137470346852TruSeq Adapter, Index 11 (100% over 49bp)
ATCGTATAGTGGTTAGTACTCTGCGTTAGATCGGAAGAGCACACGTCTGA3527010.792032823615509Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC3352070.7527479272972968Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC3033030.6811036302733893No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC2873930.6453757978495438Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT2674450.6005801472404381Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA1917880.43068318824038265No Hit
ATCGTATAGTGGTTAGTACTCTGCGAGATCGGAAGAGCACACGTCTGAAC1871800.42033536600222554Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACCTCGGGCCGATCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1859370.4175440589184518Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCAGCGATCCGAGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTC1484740.33341635394707997Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG1454610.32665029743588914Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC1405300.315577139567757Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACCCCTCCCACGGCGCGACCAGATCGGAAGAGCACACGTCTGAACTCCAG1331780.2990673329065306Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC1252970.28136959265936984Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA1188140.2668112307735251Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATCGTATAGTGGTTAGTACTCTGCGTAGATCGGAAGAGCACACGTCTGAA1117900.2510379878480008Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1101030.24724962497565464Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC1064550.23905759903711357Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACCCCTCCCACGGCGCGACCGCTCTAGATCGGAAGAGCACACGTCTGAAC1054930.23689731149520665Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA1047160.2351524638652049Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TTCCGGCTCGAAGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA981590.220427926014598Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATCCGGCTCGAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC974840.21891213174143045Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
TGCGAGAGGTCCCGGGTTCAGATCGGAAGAGCACACGTCTGAACTCCAGT963770.2164262291334357Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATTCGAACGTCTGCCCTATCAGATCGGAAGAGCACACGTCTGAACTCCAG952950.21399646705407674Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT935370.21004866508040482Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTC889780.1998108782783739No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG853720.191713168427941No Hit
ACCCCTCCCACGGCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG850310.19094741161734824Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACCCCTCCCACGGCGCGACAGATCGGAAGAGCACACGTCTGAACTCCAGT840840.1888208083926228Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG798360.17928140976682166Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA789750.1773479299606035Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTCCGGCTCGAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC781110.175407713290949Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATCCGGCTCGAAGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA767700.17239633533492282Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCA729080.163723746471259Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTCACG713430.16020934937865572Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCA701280.15748091968695413Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT670200.1505015291669471Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCAGATCGGAAGAGCACACGTCTGAACT656040.1473217296250134Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA641590.14407680706986212Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG579750.1301898859065019Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC573710.1288335307346601Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA541900.12169020987103644No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT540850.12145441965076595No Hit
ACCCCTCCCACGGCGCGACCGCTAGATCGGAAGAGCACACGTCTGAACTC536910.12056964491946517Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TTCCGGCTCGAAGGACCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC532300.11953441357142036Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCGTATAGTGGTTAGTACTCTGAGATCGGAAGAGCACACGTCTGAACTC519470.11665328164182931Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT508490.11418758962414342No Hit
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC506570.1137564303642202Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATCCGAGTCACGGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGG496380.11146814241702357TruSeq Adapter, Index 11 (100% over 35bp)
ATAAACGATGCCGACCGGCAGATCGGAAGAGCACACGTCTGAACTCCAGT459790.10325141464588272Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGTC457680.10277758858419628Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACGGAGTCGACCCGCAACGCGGAGATCGGAAGAGCACACGTCTGAACTCC452910.10170642729782453Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACCCCTCCCACGGCGCGACCGAGATCGGAAGAGCACACGTCTGAACTCCA451870.10147288269869946Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AAGGGCGCACGGTGGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA449230.10088003871630503Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGGTCTAGCGGTTAGGATTCCTGGTAGATCGGAAGAGCACACGTCTGAA448250.10065996784405257Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AACCTCGGGCCGATCGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTC447740.10054544116563546Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)