FastQCFastQC Report
Thu 28 Sept 2023
SRR5859397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5859397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23143093
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5929592.5621424068079404Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACG3396011.4673967736291773No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGT3360431.4520228562361996No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGT2737731.182957697141No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCT2496061.0785334527238861Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGAGATCGGAAGAGCACACGTC2490701.076217426944618No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC2483851.0732575805662623No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG2384341.0302598706231703TruSeq Adapter, Index 6 (100% over 49bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT2301120.9943009778338616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG1810000.7820907948648004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC1788670.7728742221275263Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
CGTGGGGGGGCCGGGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1725590.7456177097849453Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA1371180.5924791470180758No Hit
AATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAACTCCAGT1330530.5749145112107531Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1241450.5364235454612744Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC1169520.5053429980167301No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1105230.47756365149636654No Hit
GTCTAGCGGTTAGGATTCCTGGAGATCGGAAGAGCACACGTCTGAACTCC1045010.4515429290285443Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
AATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTC1000240.43219806445059006Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA956550.4133198617833839No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTG932210.4028026850170805Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGTATAGTGGTTAGTACTCTGCAGATCGGAAGAGCACACGTCTGAACT900680.389178749789408Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GTCTAGGGGTATGATTCTCGCTTAGATCGGAAGAGCACACGTCTGAACTC867100.37466902112003786Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CCGTCTCCGCCCCCCGGCCCCGCGAGATCGGAAGAGCACACGTCTGAACT800540.3459088203983798Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACA777960.33615212970885094No Hit
CCGTCTCCGCCCCCCGGCCCAGATCGGAAGAGCACACGTCTGAACTCCAG736210.3181121901035441Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA714400.3086882120726041Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGAGATCGGAAGA704230.3042938124130599No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC640070.2765706381597309Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA620190.26798060224707215Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTG593110.2562794869294264Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGGAGATCGGAAGAGCACACG588850.25443876494814244No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA584450.25253754975620585Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCAGATCGGAAGAGCACACGTCTGAA582750.2518029893411395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAAC572130.2472141472187836Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC567590.24525243881619455Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCAGATCGGAAGAGCACACGT518140.22388537262499875No Hit
GACCTGGTATTGCAGTACCTCCAGGAGATCGGAAGAGCACACGTCTGAAC476520.20590160528672638Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT457170.19754057938582364No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC448980.19400172656265088No Hit
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA447840.19350913899019462Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGAGATCGGAAGAGCACACGTCTG442860.19135730906841192Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC441840.19091657281937205Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCC437880.1892054791466292Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCAC436830.18875178006673526No Hit
ACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGAA417700.18048581492542937Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGAGACCGGGGTTCGATTCCCCGACGGGAGATCGGAAGAGCACACGTCT415330.17946175128795447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCAGATCGGAAGAGCA398850.17234083620542856No Hit
CTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAAC390100.16856001053964567Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACAC383350.1656433735974703No Hit
TTCCGGCTCGAAGGACCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA382200.165146465081396Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CTCGACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTC376350.16261871306484402No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCA357600.15451694378102357No Hit
TTCCGGCTCGAAGGACAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC352350.15224844838155382Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC350420.1514145062632726Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCGGCCCGGGTTCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTC341130.14740034964211568No Hit
GGGCGCGCGGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCT330850.14295841960277306Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGGGCGGCGGCGGTCGGCGGGCGGCGGGGCGAGATCGGAAGAGCACACGT321090.13874117863156837No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT320990.13869796919538802Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC319520.1380627904835365Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCAGATCGGAAGAG307530.13288197908550944No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA306190.1323029726406924Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGTCTAGTGGTTAGGATTAGATCGGAAGAGCACACGTCTGAACTCCAG304920.13175421280120164Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
CCGTCTCCGCCCCCCGGCCCCGCGTAGATCGGAAGAGCACACGTCTGAAC303460.13112335503296815Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGAGGGCGCGCGGGTCGGGGCGGCGGCAGATCGGAAGAGCACACGTCTG298990.12919189323570535Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCTCGCTTCTGGCGCCAAGCGCCCGGAGATCGGAAGAGCACACGTCTGAA274290.11851916249915255Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT270210.11675621750299323Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCTGGTCCGAAGGTAGTGAGTTATCTAGATCGGAAGAGCACACGTCTGAA256700.11091862267502448Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTGGTCTAGTGGTTAGGATTCGGAGATCGGAAGAGCACACGTCTGAACTC252590.10914271484801102Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA249990.10801926950732126Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGGGGGCCGGGCCACCCCTCCCACGGCGCAGATCGGAAGAGCACACGTC248110.10720693210713018No Hit
TCGATTCCCGGTCAGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCA240640.10397918722445612Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGGTCGGGGCGGCGGCGGCGGAGATCGGAAGAGCACACGTCTGAACTCCA238530.1030674681210502Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACT236010.10197859032930474Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA234770.10144279332066808No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)