FastQCFastQC Report
Thu 28 Sept 2023
SRR5859408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5859408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51484285
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG576075311.189342534328679TruSeq Adapter, Index 6 (100% over 49bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT5006460.972424886545477No Hit
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG4273890.8301348654254401Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC4245830.8246846586293273No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC3225150.6264338720057975No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA3020110.5866081271207321Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG2774490.5389003654221866No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACG2232780.4336818506851168No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC2106700.40919282456772976No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT2073580.4027597935952689Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT1713780.33287439069999714Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC1658300.32209828688501746TruSeq Adapter, Index 6 (100% over 50bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG1584860.3078337399460826No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT1569040.30476095763979244No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA1521530.29553289901957464No Hit
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA1396190.2711876060821278Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC1285280.24964511015351576Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAAC1112460.21607758561665952Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACGT1093320.2123599463409077No Hit
AGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACG1082740.2103049503358161No Hit
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC1057940.20548794646754828Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGAGATCGGAAGAGCACACGTC1035270.2010846610766761No Hit
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG1029190.19990371819284272Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG930890.18081051334402332TruSeq Adapter, Index 6 (100% over 50bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG910360.1768228887708162Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACG902530.1753020363398268No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC896280.17408807367141255TruSeq Adapter, Index 6 (100% over 50bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAACAGATCGGAAGAGCACACGT877980.17053359097829562No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC850940.16528150288966817Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCGGCCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG828730.1609675651511913No Hit
ACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTGA819050.15908737977035128Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGTC801610.15569993834040813No Hit
GCACGAGCGCACGTGTTAGGACCCGAAAAGATCGGAAGAGCACACGTCTG769670.14949610351974393Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA765770.14873859081465346Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGAGATCGGAAGAGCACACGT714640.13880740501689012No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCTG705810.13709231855895443Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGA686460.13333389013754393Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AGTGCGGTAACGCGACCGATCCCGGAGAAGATCGGAAGAGCACACGTCTG637980.12391742451118821Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGAGATCGGAAGAGC617630.1199647620628314No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC610080.118498295159387No Hit
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA592870.1151555275556415Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGGCAGGGCGCCCTGGAATGGGTTAGATCGGAAGAGCACACGTCTGAA589940.1145864218566889Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTAGATCGGAAGAGCACACGT588510.11430866719815572No Hit
AACGATGCCGACCGGCGATGCGGCGGCAGATCGGAAGAGCACACGTCTGA576310.11193901206941108Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAAAGATCGGAAGAGCACACGTC574630.11161269890414133No Hit
CTCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCT547920.10642470804440617Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGAGCGAGAAAACCAATGTTAAGGAACAGATCGGAAGAGCACACGTC530640.10306834405877444No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGAGATCGGAAGA529960.10293626492045096No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGGAGATCGGAAGAGCACACGT521590.1013105261149106No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)