FastQCFastQC Report
Thu 28 Sept 2023
SRR5859409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5859409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35036181
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG23595676.734658095298686TruSeq Adapter, Index 11 (100% over 49bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC4975601.4201319487417878Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA2104580.6006876148972972Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGAGATCGGAAGAGCA1810920.5168714021656642No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC1685400.48104557971087086No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACA1559810.44519977790958437No Hit
AGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTC1477550.4217211915876333Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACCTCGGGCCGATCGCACAGATCGGAAGAGCACACGTCTGAACTCCAGT1299050.37077385802978924Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC1259680.35953690272350175No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGGAGATCGGAAGAGC1166900.3330557060428475No Hit
ACCCCTCCCACGGCGCGACCGCTCAGATCGGAAGAGCACACGTCTGAACT896150.2557784479992269Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG821160.23437485952021997Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA819680.2339524390515051Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCAC770790.21999829262213255No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAAAGATCGGAAGAGCACACGTCT749640.21396167578880812Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC717850.2048881982885064No Hit
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC660440.188502279971667Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG659530.18824254846725447No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAACAGATCGGAAGAGCACACGT649510.18538264772636037No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG644080.1838328212769537No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACA559760.15976627132962923No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT555880.1586588446954307Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG553030.1578453998739189Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGATCGGAAGAGCACA547830.1563612198487044No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT503460.143697168364326No Hit
GCGGCCTCAGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACAC492200.14048334777126537No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC478940.13669868870696836No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA464540.13258865171406667No Hit
AGACGTGGCGACCCGCTGAATAGATCGGAAGAGCACACGTCTGAACTCCA457080.1304594242163551Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
ACCCCTCCCACGGCGCGACCGCAGATCGGAAGAGCACACGTCTGAACTCC450730.12864701207017967Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACAGATCGGAAGAGCAC424100.12104629782566771No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT411340.11740434837917979No Hit
AACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCTGAACT407980.11644533974750272Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGA405460.11572608327374494Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC395420.11286047414813846No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCA390790.11153898308722632No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGATCGGAAGAGCACACGTCTGAAC386720.11037732679826034Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC380030.10846787211197476TruSeq Adapter, Index 11 (100% over 50bp)
AGTTGGTCCGAGTGTTGTGGGTTAGATCGGAAGAGCACACGTCTGAACTC379720.10837939214893312Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AGACGTGGCGACCCGCTGAATTAGATCGGAAGAGCACACGTCTGAACTCC370040.1056165339481492Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC362620.10349872321986234TruSeq Adapter, Index 11 (100% over 50bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCTG355910.1015835601488644Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCAGACGTGGCGACCCGCTGAAAGATCGGAAGAGCACACGTCTGAAC350470.10003087950710152Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)