FastQCFastQC Report
Thu 28 Sept 2023
SRR5859410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5859410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46789389
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG45536739.732277119498184TruSeq Adapter, Index 12 (100% over 49bp)
AACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCTG2206360.47155135964694905Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTGAACCCCATTCGTGATGGGGATCGGGGATTAGATCGGAAGAGCACAC2058590.43996941272304285No Hit
GACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTGAA1858480.397201168837661Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAACAGATCGGAAGAGCACACGT1254030.2680158956553162No Hit
AGTCCGCCCGGAGGATTCAACCCGGCGGCGGGAGATCGGAAGAGCACACG1128070.24109526200481055No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGGAGATCGGAAGAGCACACGTCTG1109850.23720121671176342Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC929890.19873950480524546TruSeq Adapter, Index 12 (100% over 50bp)
AGCCCGTGGACGGTGTGAGGCCGGTAGATCGGAAGAGCACACGTCTGAAC905020.1934241970973376Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAGATCGGAAGAGCACACGT836270.1787306946880627No Hit
AAACGATGCCGACCGGCGATGCGGCGGCGAGATCGGAAGAGCACACGTCT809840.17308197805275893Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGATTTAGCTCAGCGGTAGAGCAGCTGAGATCGGAAGAGCACACGTCT788610.16854462450877483Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACAGATCGGAAGAGCACACGTCTGAA749960.16028420460886977Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGT716020.15303042320129462No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTCT686230.1466635950300612Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCGAGCGAGAAAACCAATGTTAAGGAACAGATCGGAAGAGCACACGTC652670.13949102861762097No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC652400.13943332322634092TruSeq Adapter, Index 12 (100% over 50bp)
GACTGCATAATTTGTGGTAGTGGGGAGATCGGAAGAGCACACGTCTGAAC643540.13753973149766927Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAAAGATCGGAAGAGCACACGTC636310.13599450935339205No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGAGATCGGAAGAGCACACGTC624300.1334276880597864No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAAGATCGGAAGAGCACACGTC594760.12711429080640485No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACACG594610.12708223225569368No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG586220.12528909065258365TruSeq Adapter, Index 12 (100% over 50bp)
ACTGACCCGGTGAGGCGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG577710.12347030220890468Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GACTGCATAATTTGTGGTAGTGGGGGAAGATCGGAAGAGCACACGTCTGA554340.11847558000810826Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
AAGGCGAGCGAGAAAACCAATGTTAAGGAAGATCGGAAGAGCACACGTCT538940.11518423546843068Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAAGATCGGAAGAGCACAC531130.11351505359473704No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGAGATCGGAAGAGCACA510560.109118757673882No Hit
AAGGCGAGCGAGAAAACCAATGTTAAGAGATCGGAAGAGCACACGTCTGA508130.10859940915236145Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCGACTGCATAATTTGTGGTAGTGGGGGAGATCGGAAGAGCACACGTCTG501550.10719310739449922Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACCCGGCGGCGGGTCCGGCCGTGAGATCGGAAGAGCACACGTCTGAACT477420.10203595520343299Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGATCATCGACACTTCGAACGCAAGATCGGAAGAGCACACGTCTGAAC471380.10074506422813087Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)