FastQCFastQC Report
Tue 4 Jul 2023
SRR5861928.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861928.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37525436
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA764087620.361858020783554RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA665152117.725366335517062RNA PCR Primer, Index 1 (100% over 22bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTT16587424.420313730665248RNA PCR Primer, Index 3 (100% over 35bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG9428992.512692990429212Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC4421921.1783793797892181RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG3637000.9692092584880293Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC3120210.8314920045166164RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA2912970.7762654643106612RNA PCR Primer, Index 1 (100% over 22bp)
AGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA2364740.6301698932958434RNA PCR Primer, Index 3 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2262530.6029323683274459No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG2225780.5931390110963667No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT2162900.576382377009557RNA PCR Primer, Index 1 (100% over 25bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG1933070.5151359200729874No Hit
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT1929950.514304484030512RNA PCR Primer, Index 1 (100% over 25bp)
AGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1540590.41054552970417185RNA PCR Primer, Index 1 (100% over 33bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA1372190.3656693022833899RNA PCR Primer, Index 3 (100% over 36bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG1334970.35575069667411724Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC1175940.3133714422398716No Hit
AACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGC1162300.3097365744131527RNA PCR Primer, Index 3 (100% over 39bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1161020.309395472447009Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA1136900.30296783227248847RNA PCR Primer, Index 1 (100% over 22bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA1135050.3024748333370464RNA PCR Primer, Index 1 (100% over 23bp)
AACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG1127060.3003456109077587RNA PCR Primer, Index 3 (100% over 38bp)
TTTGGTGACTCTAGATAACCTCGGGTGGAATTCTCGGGTGCCAAGGAACT972350.2591175756092481RNA PCR Primer, Index 1 (100% over 25bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA971170.25880312223420937RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC855400.22795204831197696No Hit
CTTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAA849030.22625453305858992RNA PCR Primer, Index 1 (100% over 23bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC764420.2037071601246685No Hit
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATC753440.200781144821342RNA PCR Primer, Index 3 (100% over 42bp)
AGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT727800.19394844606202577RNA PCR Primer, Index 3 (100% over 34bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG714100.19029758908064387Illumina Small RNA Adapter 2 (100% over 21bp)
TAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACT701410.18691588286942223RNA PCR Primer, Index 3 (100% over 34bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC695550.18535427543067057No Hit
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA687400.18318241525561488RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG673120.1793769964458241No Hit
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT653310.1740979105479281RNA PCR Primer, Index 1 (100% over 30bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG613200.1634091606557216No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA601590.16031525922843376RNA PCR Primer, Index 1 (100% over 22bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG598590.15951580149528444No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA579230.1543566342573608No Hit
ACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA579110.15432465594803482RNA PCR Primer, Index 3 (100% over 40bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC551080.14685505586130965RNA PCR Primer, Index 1 (100% over 26bp)
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA550750.1467671155106632RNA PCR Primer, Index 1 (100% over 23bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC546590.14565853412069615RNA PCR Primer, Index 1 (100% over 26bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA540520.14404096464062402RNA PCR Primer, Index 1 (100% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG540500.14403563492240304Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA520560.13872190585607055No Hit
GATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTA518620.13820492318863398RNA PCR Primer, Index 3 (100% over 36bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT513090.13673125610052872RNA PCR Primer, Index 1 (100% over 25bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCT507590.13526558358975496RNA PCR Primer, Index 3 (100% over 43bp)
CTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC507530.13524959443509196RNA PCR Primer, Index 1 (100% over 33bp)
TGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA504030.13431689374641778RNA PCR Primer, Index 1 (100% over 28bp)
ATGTACACGGAGTCGAGCTCAACCCGTGGAATTCTCGGGTGCCAAGGAAC502850.13400244037137904RNA PCR Primer, Index 1 (100% over 24bp)
ATGTACACGGAGTCGAGCTCAACCCGCTGGAATTCTCGGGTGCCAAGGAA491670.13102312788584256RNA PCR Primer, Index 1 (100% over 23bp)
TTTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGG489480.13043952374064355RNA PCR Primer, Index 3 (100% over 38bp)
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG460850.12281003210728851Illumina Small RNA Adapter 2 (100% over 21bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG439620.1171525362157018Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC436550.116334424468779No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA423960.11297936684866233No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC414060.11034115632926955RNA PCR Primer, Index 1 (100% over 27bp)
TGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA413930.11030651316083309RNA PCR Primer, Index 1 (100% over 32bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGA413290.11013596217776124RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC385420.10270899983680402No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC376070.10021735656848864No Hit
TTTGGTGACTCTAGATAACCTTGGGCCTGGAATTCTCGGGTGCCAAGGAA375600.10009210819029524RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)