FastQCFastQC Report
Tue 4 Jul 2023
SRR5861929.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861929.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38337818
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA560196214.612104423887661RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA473820712.359094093461447RNA PCR Primer, Index 1 (100% over 22bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAT17047354.446614567370527RNA PCR Primer, Index 1 (100% over 35bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG7398691.929867265789618Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC5741051.4974900240801394RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC4171371.0880561851485653No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG4039181.0535758712193792No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG3430180.8947248901854561Illumina Small RNA Adapter 2 (100% over 21bp)
AGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC2088010.5446345433639442RNA PCR Primer, Index 1 (100% over 36bp)
AGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2052330.5353278060843212RNA PCR Primer, Index 1 (100% over 33bp)
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT2044200.5332071846133758RNA PCR Primer, Index 1 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG2010270.5243569156700572Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA1967500.5132008295307782RNA PCR Primer, Index 1 (100% over 22bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGG1891990.4935048729168676No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1767460.4610225861054481No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG1674350.43673586222356214No Hit
AACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC1667230.4348786881924266RNA PCR Primer, Index 1 (100% over 38bp)
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC1612680.4206499180521959RNA PCR Primer, Index 1 (100% over 24bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG1538280.40124349278302696No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC1483980.3870799324051254No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1457560.3801885647221759No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG1411360.36813780064374035No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT1409500.36765264001201114RNA PCR Primer, Index 1 (100% over 25bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1397390.3644938791247848No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG1376080.3589353989838441No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1344780.3507711367402287No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1327900.3463681735877613No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1283460.33477648623612327No Hit
AGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1272040.3317977042929256RNA PCR Primer, Index 1 (100% over 34bp)
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA1182300.30839000800723715RNA PCR Primer, Index 1 (100% over 23bp)
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA1173950.30621200194544196RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG1144820.29861376043884397No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTGGAATTCTCGGGTGC1135160.2960940552224438No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA1117210.2914119942872075RNA PCR Primer, Index 1 (100% over 22bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA1034720.26989538110906575RNA PCR Primer, Index 1 (100% over 22bp)
AACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACG1016380.26511159294459585RNA PCR Primer, Index 1 (100% over 39bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA969360.2528469408457205RNA PCR Primer, Index 1 (100% over 22bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATC952470.24844136930276RNA PCR Primer, Index 1 (100% over 42bp)
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGGG923050.24076748447186014No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG891380.2325067117799975Illumina Small RNA Adapter 2 (100% over 21bp)
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCT842610.21978559134481782RNA PCR Primer, Index 1 (100% over 43bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG815620.21274554540375773No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG784740.20469083556085535No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA783380.20433609445378453RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG782520.20411177287137208No Hit
TGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA770630.20101039657499548RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA762520.19889499188503632No Hit
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA761900.19873327167445992RNA PCR Primer, Index 1 (100% over 22bp)
TAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA742700.1937251619275776RNA PCR Primer, Index 1 (100% over 34bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG709850.18515659915752117Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA687090.1792199023950711No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG636210.16594841156583298Illumina Small RNA Adapter 2 (100% over 21bp)
TGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA608010.1585927503750996RNA PCR Primer, Index 1 (100% over 28bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC603550.15742940821514673No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG581310.1516283477583414No Hit
GATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATC562330.14667762260230877RNA PCR Primer, Index 1 (100% over 36bp)
TTTGGTGACTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT554860.14472915490391236RNA PCR Primer, Index 1 (100% over 25bp)
TGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAAC551300.1438005678883446RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTTGGGCCTGGAATTCTCGGGTGCCAAGGAA551170.14376665881193343RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA544750.14209207211531966No Hit
ACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGA539520.14072788388739285RNA PCR Primer, Index 1 (100% over 40bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG492110.128361504559284Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC491120.12810327390046036No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG478150.12472019143082165No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC474640.12380464636771973No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGTGC470590.12274824821798674No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC466170.12159533962000654No Hit
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC466130.12158490605803386RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG462670.1206824029473978No Hit
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT453980.11841571160883492RNA PCR Primer, Index 1 (100% over 25bp)
CTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC449640.11728367013480005RNA PCR Primer, Index 1 (100% over 33bp)
AACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGGTGCCAA449450.11723411071542986No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG449040.11712716670520998Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG444960.11606294338399749Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA431960.11267203574287926No Hit
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT420630.10971672931412008RNA PCR Primer, Index 1 (100% over 30bp)
TAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCAC415940.10849339417282433RNA PCR Primer, Index 1 (100% over 38bp)
CTTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAA411800.10741352050865284RNA PCR Primer, Index 1 (100% over 23bp)
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGGGT394550.10291404690793828No Hit
ATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCA393290.10258538970579913RNA PCR Primer, Index 1 (100% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)