FastQCFastQC Report
Tue 4 Jul 2023
SRR5861930.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861930.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38469119
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA610471715.869136488412952RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA473478712.308020363034569RNA PCR Primer, Index 1 (100% over 22bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACAC18765194.877987977837496RNA PCR Primer, Index 5 (100% over 35bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG7217291.8761256268956927Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC6046711.5718348007917728RNA PCR Primer, Index 1 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC4638171.2056865664118797No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG3203720.8328030595137882No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG2817420.7323848513401099Illumina Small RNA Adapter 2 (100% over 21bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT2450990.6371318251400558RNA PCR Primer, Index 1 (100% over 25bp)
AGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA2373810.6170689794065728RNA PCR Primer, Index 5 (100% over 36bp)
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT2349550.6107626223517102RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC2259150.5872632539362287RNA PCR Primer, Index 1 (100% over 24bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1839580.47819655032910946No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA1698700.441574968223213RNA PCR Primer, Index 1 (100% over 22bp)
AGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC1693540.4402336325924178RNA PCR Primer, Index 1 (100% over 33bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1692700.4400152756292651No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG1637620.4256972976168235No Hit
TCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCT1629040.4234669372074781RNA PCR Primer, Index 5 (100% over 43bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATC1607090.41776106180128536RNA PCR Primer, Index 5 (100% over 42bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1572850.40886041606515605Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC1447770.3763460244566557No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG1362690.3542295834744747No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG1315460.34195220327244824No Hit
AACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT1304090.33899658580691694RNA PCR Primer, Index 5 (100% over 38bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA1291180.33564064724227244No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG1235390.32113810560621364No Hit
TAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA1088020.2828294560111969RNA PCR Primer, Index 1 (100% over 34bp)
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA1055280.2743187334235546RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1032070.2682853225726328No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA1020990.26540509024914244RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC998470.2595510440465247No Hit
ACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGA972640.25283656742957905RNA PCR Primer, Index 5 (100% over 40bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG967960.2516200072062997No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA950250.24701631456649684No Hit
AACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTG947580.24632225136219005RNA PCR Primer, Index 5 (100% over 39bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA947180.24621827185592685RNA PCR Primer, Index 1 (100% over 23bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC911550.23695629733553294RNA PCR Primer, Index 1 (100% over 26bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG906340.23560196426645488No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG902700.23465575075945982No Hit
AGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACA898460.23355356799306995RNA PCR Primer, Index 1 (100% over 34bp)
TGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCA895420.23276332374546968RNA PCR Primer, Index 1 (100% over 28bp)
GTCTACGGCCATACCACCCTGAACGCGCCCGATGGAATTCTCGGGTGCCA871510.2265479487585874No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA862060.22409143292311945RNA PCR Primer, Index 1 (100% over 22bp)
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG806330.20960448821300015No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG802700.2086608741936617Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA739760.19229969888314832RNA PCR Primer, Index 1 (100% over 22bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT736900.1915562454133665RNA PCR Primer, Index 1 (100% over 25bp)
CTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC717720.1865704280880464RNA PCR Primer, Index 1 (100% over 33bp)
GATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA713900.18557742380323292RNA PCR Primer, Index 5 (100% over 36bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC713440.18545784737103024RNA PCR Primer, Index 1 (100% over 26bp)
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA663850.17256698808205095RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA626650.16289689399957405RNA PCR Primer, Index 1 (100% over 22bp)
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC608970.15830099982274093RNA PCR Primer, Index 1 (100% over 27bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG606810.1577395104889197Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTTGGGCCTGGAATTCTCGGGTGCCAAGGAA597640.155355780307836RNA PCR Primer, Index 1 (100% over 23bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG581630.15119400056965174Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT547930.14243372716697775RNA PCR Primer, Index 1 (100% over 25bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA539180.14015917546747042RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC537520.13972766051647817No Hit
TCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA528260.13732053494648527RNA PCR Primer, Index 1 (100% over 32bp)
GTCTACGGCCATACCACCCTGAACGCGCCTGGAATTCTCGGGTGCCAAGG510720.13276103359684427Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA508020.1320591719295677No Hit
TGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA506810.13174463392312155RNA PCR Primer, Index 1 (100% over 32bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA502490.13062165525547909No Hit
CGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTCG494840.12863304719819552RNA PCR Primer, Index 5 (100% over 45bp)
AGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA491640.12780121114808998RNA PCR Primer, Index 5 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG484510.12594777644894858Illumina Small RNA Adapter 2 (100% over 21bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACA472850.1229167738413765RNA PCR Primer, Index 5 (100% over 36bp)
GTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCC468060.12167161925387478RNA PCR Primer, Index 1 (100% over 27bp)
CCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGTGATCTC462610.12025489848103879RNA PCR Primer, Index 5 (100% over 44bp)
CTTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAA458770.11925669522091215RNA PCR Primer, Index 1 (100% over 23bp)
GGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTC456690.11871600178834352RNA PCR Primer, Index 1 (100% over 26bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGGAATTCTCGGGTGCCAAG447140.11623349107630981No Hit
TGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAAC443370.11525348422977923RNA PCR Primer, Index 1 (100% over 24bp)
TAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAGT435760.11327527412312198RNA PCR Primer, Index 5 (100% over 38bp)
TTTGGTGACTCTAGATAACCTCGGGTGGAATTCTCGGGTGCCAAGGAACT435270.11314789922794957RNA PCR Primer, Index 1 (100% over 25bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGA424090.11024167202789334RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC419200.10897052256382582No Hit
TAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTC405400.10538322959774567RNA PCR Primer, Index 1 (100% over 31bp)
ATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACACAG404000.10501930132582449RNA PCR Primer, Index 5 (100% over 37bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC391590.10179333714400894RNA PCR Primer, Index 1 (100% over 24bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA391140.10167636019946284RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)