FastQCFastQC Report
Tue 4 Jul 2023
SRR5861931.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861931.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41158199
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA825829820.06477008384162RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA567325713.784026361308957RNA PCR Primer, Index 1 (100% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG9574232.326202368573027No Hit
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCT7714131.8742632543275277RNA PCR Primer, Index 12 (100% over 35bp)
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC5407721.3138864506680674RNA PCR Primer, Index 1 (100% over 24bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC4353681.057791668678214No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC3903950.948523039115487No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG3416420.8300703342242939Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG3228780.7844803899218233Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA2845350.6913203369272791RNA PCR Primer, Index 1 (100% over 22bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC2504980.6086223549286013No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC2348330.5705618946057383RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2312460.5618467416419266No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2212710.5376109873029187No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG1949780.4737282114798074Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1637590.3978769819349967No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1550180.3766394151503082Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG1463810.35565453191963037No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG1415190.34384157576962976Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGTGGAATTCTCGGGTGCCAAGGAACT1278490.31062826631456836RNA PCR Primer, Index 1 (100% over 25bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1177950.2862005696604946No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC1143260.2777721153445028RNA PCR Primer, Index 1 (100% over 24bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCA1140230.2770359315284908No Hit
TTTGGTGACTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT1030630.2504069723750546RNA PCR Primer, Index 1 (100% over 25bp)
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA1029920.25023446725645115RNA PCR Primer, Index 1 (100% over 23bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG887060.2155244936737878Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGA874490.21247042417963913RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA845320.20538313641955036No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC820500.19935274621710247No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA776280.18860883587253174RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC765810.18606499278552008RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA758410.18426705211275157No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG724980.17614473364104197No Hit
AACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTA721460.17528949699669802RNA PCR Primer, Index 12 (100% over 39bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC718320.17452658703555032No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG671290.16309994516523912No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG661370.16068973280390622No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC633440.15390372158898402No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGGA625780.15204261002771283RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC623480.1514837906294199No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC611270.14851718851935186No Hit
GAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAACTC579830.14087837030964354RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGTGACTCTAGATAACCTTGGGCCTGGAATTCTCGGGTGCCAAGGAA562740.1367260992153714RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA520020.12634663630446996No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA518850.12606236730620793No Hit
TTTGGTGACTTTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA499850.1214460331463969RNA PCR Primer, Index 1 (100% over 23bp)
TGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAGG475490.1155274068236076Illumina Small RNA Adapter 2 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA467240.11352294593842652RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC457640.11119048236294304No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG456840.11099611039831943Illumina Small RNA Adapter 2 (100% over 21bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG442440.10749741503509422No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC441100.10717184199434966RNA PCR Primer, Index 1 (100% over 24bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCCAAGGAA434680.10561200697824509RNA PCR Primer, Index 1 (100% over 23bp)
TTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGCCAAGG429030.10423925497809075Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA422300.10260410082569453RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCCAA416230.1011293035441128No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)