FastQCFastQC Report
Tue 4 Jul 2023
SRR5861932.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861932.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35992488
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA899901125.00246995984273RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA423816911.775148747705355RNA PCR Primer, Index 1 (100% over 22bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTG10160212.822869594344242RNA PCR Primer, Index 4 (100% over 35bp)
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC6166841.7133686340327459RNA PCR Primer, Index 1 (100% over 24bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG3819721.061254781831142No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC2559360.7110817123839841No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC2548420.7080421892479342RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA2497520.6939003494284697RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG2284280.6346546534932511Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2024270.5624145793977898No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC1780310.4946337691353818No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA1591690.44222838943503995No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1578530.4385720709276892No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC1541850.4283810555135838No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG1411650.39220684049404975Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1408790.39141223024093247No Hit
AACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCA1404260.3901536342805754RNA PCR Primer, Index 4 (100% over 39bp)
TTTGGTGACTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT1360350.37795386637344996RNA PCR Primer, Index 1 (100% over 25bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1281510.35604929561968596Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG1269040.3525846837817936No Hit
TTTGGTGACTCTAGATAACCTCGGGTGGAATTCTCGGGTGCCAAGGAACT1240780.34473304540658595RNA PCR Primer, Index 1 (100% over 25bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG1015480.28213665029213875Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA934580.2596597378875281RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC848310.2356908474901763No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG808960.22475801061599296Illumina Small RNA Adapter 2 (100% over 21bp)
CGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAATCTC753550.20936313155122815RNA PCR Primer, Index 4 (100% over 44bp)
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC740510.20574015333421797RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC706110.19618260343658378No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCA690740.1919122679154606No Hit
TTTGGTGACTCTAGATAACCTCGGGCCTTTGGTGACTCTAGATAACCTCG669220.18593324251438245No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG645530.1793513135296454Illumina Small RNA Adapter 2 (100% over 21bp)
AGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGA612230.17009938296013324RNA PCR Primer, Index 4 (100% over 36bp)
TTTGGTGACTTTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA610160.16952426295175815RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA604860.16805173346171567No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA593450.16488162752183178No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG593070.16477604993575326No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA584430.16237554903123116No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC581150.1614642477619219No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGA578480.16072242630184388RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACCAA572000.15892205062345233RNA PCR Primer, Index 4 (100% over 40bp)
TTTGGTGACTCTAGATAACCTTGGGCCTGGAATTCTCGGGTGCCAAGGAA548500.15239291043175454RNA PCR Primer, Index 1 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA537810.14942284623391414RNA PCR Primer, Index 1 (100% over 23bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG522030.1450385980541273No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAGG482340.13401129702397901Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGCTTCTGGCGCCAAGCGCCCGGCTGGAATTCTCGGGTGCCAAGGAAC476740.13245541680808506RNA PCR Primer, Index 1 (100% over 24bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA451210.12536227003812572RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG440240.12231441182949064No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC437120.12144756428063544RNA PCR Primer, Index 1 (100% over 24bp)
GAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAACTC429040.11920265139770277RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTGACC428700.11910818724173777RNA PCR Primer, Index 4 (100% over 38bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC416240.11564635376137375No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC408690.11354869382744534No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC407050.11309304319279066No Hit
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA406460.11292912009861612RNA PCR Primer, Index 1 (100% over 22bp)
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC400210.11119264664337736No Hit
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT396690.11021466479338689RNA PCR Primer, Index 1 (100% over 25bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT393280.10926724487620862RNA PCR Primer, Index 1 (100% over 25bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA389830.10830871152891682No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGTGGAATTCTCGGGTGC383360.10651111420805365No Hit
TTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGCCAAGG365590.10157397288011877Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG360340.10011533517771819No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)