FastQCFastQC Report
Tue 4 Jul 2023
SRR5861933.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861933.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46986270
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA43593929.278012491734287RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA18068403.845463791869412RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG15636953.327982834134312No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG11218722.3876592034226167Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC10806352.299895267277015No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC7269921.5472434819788845No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC5332891.134989008491204No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG5296161.1271718312604937No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC5183361.1031648181479397No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC4678730.9957653586888255No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA4318560.9191110509516929RNA PCR Primer, Index 1 (100% over 22bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA4220810.8983071012021171RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT4137730.8806253401259561No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC3972400.8454384653218908No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC3638540.7743836656963832No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG3338960.7106246143820312No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG3235230.6885479524124813No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA3064910.6522990652375683RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA2763220.5880909465680081RNA PCR Primer, Index 1 (100% over 23bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC2689080.572311868977895No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG2592650.5517888523604875Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA2431470.5174852142977087No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAGG2360310.5023403645362783Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA2075840.4417971462727303RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG2054260.4372043152180413Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2014410.4287231142203882No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG1903830.40518857955738985No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1851310.39401084614718296No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAA1711520.36425960179431144No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG1698400.361467296723064No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1593490.33913949755960626No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC1553410.3306093460919541RNA PCR Primer, Index 1 (100% over 24bp)
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA1540400.327840452115054No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC1304810.277700272866946No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG1300090.2766957240913143Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA1296570.27594656907219917No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC1186380.2524950373800687No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG1145640.24382441934633242Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGA1034090.22008344139681657RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG984460.20952078128355367No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA977540.20804801062097503No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC971530.20676891355708807No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC947710.2016993474902349No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA932450.19845159021986636RNA PCR Primer, Index 1 (100% over 23bp)
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC904000.19239662990912026RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA900230.1915942678573975No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGTGC874970.1862182292827245No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG873750.18595857896359938No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA855170.18200423230020174No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG854600.1818829202658564No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG841700.17913743738330368No Hit
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA813570.17315058207429532RNA PCR Primer, Index 1 (100% over 23bp)
TTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGCCAAGG802390.17077116357608296Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC787190.16753617599354023RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC784620.166989207698334No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA744000.15834412904024942No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAAG715670.15231470810515496No Hit
TCCCTGGTAGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA708540.15079724353518592RNA PCR Primer, Index 1 (100% over 22bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA691930.14726216828873626RNA PCR Primer, Index 7 (100% over 35bp)
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC681780.14510196276486725RNA PCR Primer, Index 1 (100% over 24bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA681020.14494021338574012RNA PCR Primer, Index 1 (100% over 23bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGG675380.14373986273011244Illumina Small RNA Adapter 2 (100% over 21bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA674020.14345041647272702RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCCAA671340.14288003708317346No Hit
ACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGA666880.14193082362145365RNA PCR Primer, Index 1 (100% over 22bp)
GGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCA664430.1414093947018991No Hit
CCAGTGCGCCCCGGGCGGGTCGCACCGTCGGGCCCGGGGGTGGAATTCTC649680.13827017977805006No Hit
TCCCACATGGTCTAGCGGTTAGGATTCCTGTGGAATTCTCGGGTGCCAAG628520.1337667365381419No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT624160.13283880588946514No Hit
AGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTG614330.1307467053673339No Hit
GAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAACTC607990.1293973750204049RNA PCR Primer, Index 1 (100% over 26bp)
GCATTGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAGG600240.12774795700956895Illumina Small RNA Adapter 2 (100% over 21bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGA596070.12686046370567403RNA PCR Primer, Index 1 (100% over 22bp)
AGGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCC590490.12567288273787214No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG571060.12153763216360865No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT568970.12109282137100902No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGTGGAATTCTCGGGTGCCAAGGA565820.12042241276015311RNA PCR Primer, Index 1 (100% over 22bp)
CTGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAG556110.11835585161367353No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAAG543490.11566996060764134No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA515590.10973205576863199RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC503410.10713980913998919RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCA495870.10553508503654366No Hit
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC493380.10500514299177185No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGTGGAATTCTCGGGTGCCA490470.10438581313221926No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGC472640.10059108756664448No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)