FastQCFastQC Report
Tue 4 Jul 2023
SRR5861934.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861934.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42767695
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA431106510.080190199635496RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA32046677.49319550656167RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG10063502.353061113066767No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG9637912.253549086524303Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC5518621.2903711551440873No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC5246981.2268559247815436No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC4877821.1405384367803784No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC4738761.1080232404388406No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA4429871.0357981649466963RNA PCR Primer, Index 1 (100% over 22bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG3687300.862169448224881Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG3584750.8381910692170808No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC3579490.8369611689383775No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA2964550.6931750705760504RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG2756380.6445004810289635Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA2585460.6045357366114774RNA PCR Primer, Index 1 (100% over 23bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG2567020.6002240709956429Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC2269470.5306505295644294No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2260940.5286560334850873No Hit
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC2171550.5077547433875031RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC2125410.4969662264940862No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA2042530.4775871133574068RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA1996340.4667869053967019RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG1866980.43653977610904676No Hit
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC1824160.42652754608355675RNA PCR Primer, Index 1 (100% over 24bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA1783480.41701569373799546RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT1781610.4165784478214222No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA1748930.40893716624194965RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1718670.40186173231921896Illumina Small RNA Adapter 2 (100% over 21bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG1715700.4011672829223085No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT1614060.377401681339151No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAGG1572870.3677705801072515Illumina Small RNA Adapter 2 (100% over 21bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA1548930.36217289708973094No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC1515890.3544474398257844No Hit
GCCGTGATCGTATAGTGGTTAGTACTTGGAATTCTCGGGTGCCAAGGAAC1483300.34682720216743035RNA PCR Primer, Index 1 (100% over 24bp)
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG1473680.3445778408212086No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA1473530.34454276761934444No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC1472910.3443977983849726RNA PCR Primer, Index 1 (100% over 24bp)
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA1361450.3183360711864411No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG1304690.3050643716010414No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG1280730.29946201215660556Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA1274930.29810584835119125RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA1262300.2951526847542286RNA PCR Primer, Index 1 (100% over 22bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC1252480.29285655913885467No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCCA1247290.29164302635435463No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC1179330.27575252769643066No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC1155340.270143153611622RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAA1110170.2595814434235934No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGCCAAGGA1037000.24247273555425422RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGCCGACTTAGAACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGA947740.221601842231619RNA PCR Primer, Index 1 (100% over 22bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCCAA927870.21695581209134604No Hit
TTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGCCAAGG895640.209419750117466Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAA877950.20528345051095226RNA PCR Primer, Index 1 (100% over 23bp)
GGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAGG876670.20498415918837803Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC867640.20287275243615538No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG839840.19637251902399697No Hit
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT807950.1889159563076757RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA806700.18862367962547433No Hit
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA804030.1879993766322922RNA PCR Primer, Index 1 (100% over 23bp)
AGCGGTCGGCGTCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGTG800260.18711787015877288RNA PCR Primer, Index 19 (100% over 36bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG763270.17846881857907004No Hit
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA727340.17006761762587394RNA PCR Primer, Index 1 (100% over 23bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC704790.16479494627896124RNA PCR Primer, Index 1 (100% over 26bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGTGC687020.16063994096478662No Hit
GAGAATTAATGTGAATTGCAGGACTGGAATTCTCGGGTGCCAAGGAACTC665440.15559407632326222RNA PCR Primer, Index 1 (100% over 26bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAAG649020.15175472982586505No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTGC639900.14962227915252388No Hit
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA630990.14753893096179255RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC625440.14624122249281848No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAA614180.14360839413954857No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGA596570.13949080024069568RNA PCR Primer, Index 1 (100% over 22bp)
GCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAA595040.1391330535816812RNA PCR Primer, Index 1 (100% over 23bp)
TCCCTGGTAGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA582450.13618924283854905RNA PCR Primer, Index 1 (100% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG579870.13558598376648542No Hit
TTTGGTGACTCTAGATAACCTCGGGTGGAATTCTCGGGTGCCAAGGAACT571540.13363825195629553RNA PCR Primer, Index 1 (100% over 25bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC554770.12971706798788196RNA PCR Primer, Index 1 (100% over 26bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAA552900.12927982207130873No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAAC532400.1244864844832063RNA PCR Primer, Index 1 (100% over 24bp)
ACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGA515790.12060271193011454RNA PCR Primer, Index 1 (100% over 22bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGTGCC515610.12056062408787754No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGTGGAATTCTCGGGTGCCAAGGA508010.11878358186009325RNA PCR Primer, Index 1 (100% over 22bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT505680.11823877812446987RNA PCR Primer, Index 1 (100% over 25bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC504780.1180283389132849No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA494700.11567141974801307No Hit
GGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTCC485600.11354364550158712RNA PCR Primer, Index 1 (100% over 27bp)
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGGAATTCTCGGGTGCCAAG479970.11222723132495217No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCTGGAATTCTCGGGTGCCAAG467090.10921561239154928No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG458480.10720241060454626No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)