FastQCFastQC Report
Tue 4 Jul 2023
SRR5861935.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861935.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51233765
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA26610425.1939224064442655RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA18262963.5646335966134832RNA PCR Primer, Index 1 (100% over 23bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG14671162.863572489743824No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG10082191.9678799713431172Illumina Small RNA Adapter 2 (100% over 21bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC9057451.7678673429524456No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTC7312641.4273087289212494No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC6758851.3192179024906718No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC6726571.3129173700195564No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG5274771.0295495558446661No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCG4548530.8877992862714658No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG4475470.8735391591853537No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT3896210.7604770018365817No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC3794110.7405487377318454No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGG3487010.6806077983923298No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGT3424530.6684127157158956No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG3423130.6681394584216093Illumina Small RNA Adapter 2 (100% over 21bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC3369420.6576561375100971No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC3137840.6124554773595109No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGTGC2835290.5534026242264256No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAA2813130.5490773516254368No Hit
CGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCCAA2579760.5035273125057275No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAGG2573850.502373776356276Illumina Small RNA Adapter 2 (100% over 21bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG2554530.4986028256951251No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2532390.4942814567697689No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA2457560.4796758543901663RNA PCR Primer, Index 1 (100% over 22bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCCAA2071330.4042900224100259No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA2059400.4019614798951434RNA PCR Primer, Index 1 (100% over 22bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC2039570.3980909855053596No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGT1918790.37451668835971746No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGT1825980.35640168158635227No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1791180.34960928598552143No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG1659850.32397579994365044No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA1613100.31485095815230446RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA1596680.31164604045788946No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGGG1549130.302365051641237No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTG1525670.2977860401241252No Hit
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGTGCC1508230.2943820349724445No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1500230.2928205647193799Illumina Small RNA Adapter 2 (100% over 21bp)
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTCGGGTGCC1469370.28679719321818337No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA1414360.2760601333905482No Hit
GGCGAAGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTGC1372530.2678955958048369No Hit
TCCCTGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGAA1328360.25927432817010426RNA PCR Primer, Index 1 (100% over 23bp)
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGCTGGAATTCTCGGGTG1275810.24901742044528644No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA1252970.2445594228727871RNA PCR Primer, Index 1 (100% over 23bp)
GGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCA1243300.24267199570439532No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAAG1202610.23472996762974574No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG1155610.22555632989299146Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC1146170.22371379499437527RNA PCR Primer, Index 1 (100% over 24bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGCCA1043080.20359229894582215No Hit
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTGC1021430.1993665700734662No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGCC1014530.19801980198019803No Hit
AGCCTCTGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTG1010560.19724492236711474No Hit
ATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGAAC1005470.19625143691860242RNA PCR Primer, Index 1 (100% over 24bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCA967040.18875052419044355No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA965780.1885045926255859RNA PCR Primer, Index 1 (100% over 22bp)
GAGACTCTGGCATGCTAACTAGTTACGCGACCCCTGGAATTCTCGGGTGC950720.18556512487419186No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA920860.1797369371546284No Hit
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC914030.17840383192607454RNA PCR Primer, Index 1 (100% over 24bp)
GGGCGAAGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTG911220.1778553654996856No Hit
AGGACACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCC909390.17749817917929708No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC860740.16800248820284827No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC815690.15920945884027846No Hit
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT796880.15553805190776043RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA781980.1526298135614277No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCCA733830.1432317144757954No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGCC728500.14219138491969116No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGAATCTGGAATTCTCGGGTG710530.13868393236374488No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGG708070.13820378026092753Illumina Small RNA Adapter 2 (100% over 21bp)
ACATTGATCATCGACACTTCGAACGCACTGGAATTCTCGGGTGCCAAGGA703850.137380104702436RNA PCR Primer, Index 1 (100% over 22bp)
CTGGCATGTTGGAACAATGTAGGTAAGGGATGGAATTCTCGGGTGCCAAG692900.13524284229355388No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCTGGAATTCTCGGGTGCCAAG691330.13493640375638996No Hit
CCAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCCCGGGGGTGGAATTC687830.1342532605206742No Hit
CAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAA684640.1336306242572647No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCTGGAATTCTCGGGTGCCAAGGA659220.12866905252815208RNA PCR Primer, Index 1 (100% over 22bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGATGGAATTCT646230.1261336152047385No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCTGGAATTCTCGGGTGCCAA641670.12524357716049173No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGTGGAATTCTCGG640970.12510694851334858No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCTGGAATTCTCGGGT635390.12401782301183603No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCTGGAATTCTCGGGTGC633850.12371723998812112No Hit
CGAAGCTACCATCTGTGGGATTATGACTGAACTGGAATTCTCGGGTGCCA621580.12132233498748336No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAA617200.1204674300239305No Hit
TGGCATGTTGGAACAATGTAGGTAAGGGTGGAATTCTCGGGTGCCAAGGA615150.12006730327158271RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATCGCTGGAATTCTCGGGTGC609080.11888253771706997No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCA587990.11476611176242854No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA576430.11250978724675026RNA PCR Primer, Index 1 (100% over 22bp)
TCCCTGGTAGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA574400.11211356417003514RNA PCR Primer, Index 1 (100% over 22bp)
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGGG574350.11210380498095347No Hit
CGGCGTCCGGTGAGCTCTCGCTGGCCTGGAATTCTCGGGTGCCAAGGAAC569670.11119034488291071RNA PCR Primer, Index 1 (100% over 24bp)
GGCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAG568640.11098930558782866No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCTGGAATTCTCGGGTGCCAAGGAA563820.11004851976035726RNA PCR Primer, Index 1 (100% over 23bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATTGGAATTCTCGGGTG562510.10979282900641794No Hit
TTGGAACAATGTAGGTAAGGGAAGTCGGCTGGAATTCTCGGGTGCCAAGG537260.10486443852018293Illumina Small RNA Adapter 2 (100% over 21bp)
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA535410.10450334852416177RNA PCR Primer, Index 1 (100% over 23bp)
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGGAATTCTCGG535330.10448773382163111No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCTGGAATTCTCGGGTG530870.10361721415554763No Hit
CTTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAA527080.10287746762315828RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA525870.10264129524738226RNA PCR Primer, Index 1 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)