FastQCFastQC Report
Tue 4 Jul 2023
SRR5861936.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861936.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38904093
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA698997817.96720463319888RNA PCR Primer, Index 1 (100% over 22bp)
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA455313411.70348322990077RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG12343843.1728898036512505Illumina Small RNA Adapter 2 (100% over 21bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCA11499292.9558046758730505RNA PCR Primer, Index 7 (100% over 35bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG5100231.3109751716869482Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG4299981.1052770205952367No Hit
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC2821520.7252501684077303RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA2420260.6221093497797262RNA PCR Primer, Index 1 (100% over 22bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG2398580.6165366713471511No Hit
AGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT2247890.5778029576476695RNA PCR Primer, Index 34 (100% over 36bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2144820.5513096012802561No Hit
AGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC2122790.5456469580205867RNA PCR Primer, Index 1 (100% over 33bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC1718670.44177099823404187No Hit
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA1709670.43945761696590635RNA PCR Primer, Index 1 (100% over 22bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG1658150.42621479441764654No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG1616320.41546271236807913Illumina Small RNA Adapter 2 (100% over 21bp)
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1580930.4063659831370442RNA PCR Primer, Index 1 (100% over 30bp)
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC1564100.4020399601656309RNA PCR Primer, Index 1 (100% over 24bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC1428750.3672492763165048No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1280540.32915302767757626No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA1236220.31776091014382474RNA PCR Primer, Index 1 (100% over 22bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC1221650.3140158029130765No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC1135240.2918047723153448No Hit
AACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTT1099930.28272860647336RNA PCR Primer, Index 35 (100% over 38bp)
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT1063820.2734468067408743RNA PCR Primer, Index 1 (100% over 25bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG1045430.26871979768298415Illumina Small RNA Adapter 2 (100% over 21bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC1014790.26084401967679854No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC1012360.26021940673440197No Hit
AGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC1004800.2582761664691682RNA PCR Primer, Index 2 (100% over 34bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA982710.25259810066771127No Hit
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCAT951760.2446426395289565RNA PCR Primer, Index 34 (100% over 36bp)
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA945610.2430618289957306RNA PCR Primer, Index 1 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA893930.22977787966937052RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAA821240.21109347029372974RNA PCR Primer, Index 1 (100% over 23bp)
AACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTT783280.20133614218946064RNA PCR Primer, Index 35 (100% over 39bp)
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG757600.19473529430438077Illumina Small RNA Adapter 2 (100% over 21bp)
TCCCTGGTGGTCTAGTGGTTAGGATTCGTGGAATTCTCGGGTGCCAAGGA755170.1941106813619842RNA PCR Primer, Index 1 (100% over 22bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT745880.19172275780854214RNA PCR Primer, Index 1 (100% over 25bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC726720.18679782613104487No Hit
GCTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAA714980.18378014878794371No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG686410.17643644847342926No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTTGGAATTCTCGGGTGCCAAGGA682030.17531060292293668RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGATTGGAATTCTCGGGTGCCAA655030.16837045911853027No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGAATGGAATTCTCGGGTG639280.16432204189929323No Hit
ATGTACACGGAGTCGAGCTCAACCCGCTGGAATTCTCGGGTGCCAAGGAA620750.15955904691056544RNA PCR Primer, Index 1 (100% over 23bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA615940.15832267314392856RNA PCR Primer, Index 1 (100% over 22bp)
ATTCGAACGTCTGCCCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC608360.15637429203143227RNA PCR Primer, Index 1 (100% over 33bp)
TAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACC581110.14936988763624434RNA PCR Primer, Index 2 (100% over 34bp)
CTGTCCCCGCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA570120.1465449920654878RNA PCR Primer, Index 1 (100% over 32bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA566220.14554252684929578No Hit
TGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA557830.14338594142266728RNA PCR Primer, Index 1 (100% over 32bp)
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA505970.1300557244709445RNA PCR Primer, Index 1 (100% over 23bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGGAATTCTCGGGTGCCAAGGAA499200.12831554767258035RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG498640.12817160394922972No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGG472320.12140624895174912Illumina Small RNA Adapter 2 (100% over 21bp)
CTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATTTTATC450470.11578987331744246RNA PCR Primer, Index 35 (100% over 42bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC440210.11315261867176803No Hit
CTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC431680.11096004731430185RNA PCR Primer, Index 1 (100% over 33bp)
CGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAGG430020.11053335699151244Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAG422100.10849758147555323No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC403520.10372173436866913No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG401430.10318451582973544Illumina Small RNA Adapter 2 (100% over 21bp)
CTTTGGTGACTCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCATT393640.10118215582098264RNA PCR Primer, Index 35 (100% over 37bp)
CGACTCTTAGCGGTGGATCACTCGGCTTGGAATTCTCGGGTGCCAAGGAA390130.10027993712640983RNA PCR Primer, Index 1 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)