FastQCFastQC Report
Tue 4 Jul 2023
SRR5861937.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5861937.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32685660
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA621688819.02023089024361RNA PCR Primer, Index 1 (100% over 23bp)
CTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGA559643217.121979485805088RNA PCR Primer, Index 1 (100% over 22bp)
CTTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGG8267202.529304900069327Illumina Small RNA Adapter 2 (100% over 21bp)
AGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGC5361901.6404441580803324RNA PCR Primer, Index 6 (100% over 35bp)
TTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAAC4082281.2489513750066543RNA PCR Primer, Index 1 (100% over 24bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGG3345511.0235405985377073Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAAC2405310.7358915194002508RNA PCR Primer, Index 1 (100% over 24bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAG2328650.7124378091187389No Hit
CGCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAG2130250.6517384076074951No Hit
CTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAG2125100.650162793102541No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGA2050450.6273240313948073RNA PCR Primer, Index 1 (100% over 22bp)
TGGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACT1610010.49257380759635877RNA PCR Primer, Index 1 (100% over 25bp)
AGGATAGCTGGCGCTCTCGCAGACCCGATGGAATTCTCGGGTGCCAAGGA1327350.4060955171166805RNA PCR Primer, Index 1 (100% over 22bp)
ATGTACACGGAGTCGAGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGT1319750.4037703384297579RNA PCR Primer, Index 1 (100% over 30bp)
CTTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGA1229250.3760823553815343RNA PCR Primer, Index 1 (100% over 22bp)
TGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACT1179930.3609931694816626RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTGC1164080.3561439481411726No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCC1146750.35084192884586085No Hit
ATTGATCATCGACACTTCGAACGCACTTGGAATTCTCGGGTGCCAAGGAA1131320.3461212042222797RNA PCR Primer, Index 1 (100% over 23bp)
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCTGGAATTCTCGGGTGCC1081460.3308668082578109No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTTGGAATTCTC1024910.313565643159722No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGG1010080.3090284852745822Illumina Small RNA Adapter 2 (100% over 21bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGG966070.29556386500991566Illumina Small RNA Adapter 2 (100% over 21bp)
TGAAACTGCGAATGGCTCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA933360.28555641831922624RNA PCR Primer, Index 1 (100% over 32bp)
ATGTACACGGAGTCGAGCTCAACCCGCTGGAATTCTCGGGTGCCAAGGAA923080.2824113082005993RNA PCR Primer, Index 1 (100% over 23bp)
AGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC842620.25779500857562615RNA PCR Primer, Index 6 (100% over 36bp)
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTG746190.22829277426247474No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGTGGAATTCTCGGGTGCCAAGG741810.2269527370718535Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGATAACTGTGGTAATTCTAGAGCTTGGAATTCTCGGGTGCCAAGGAA729530.22319573782508903RNA PCR Primer, Index 1 (100% over 23bp)
AGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAAGGAACTCCAGTCAC727150.2224675897626054RNA PCR Primer, Index 1 (100% over 33bp)
TTTGGTGACTCTAGATAACCTCGGGTGGAATTCTCGGGTGCCAAGGAACT708830.21686268534886552RNA PCR Primer, Index 1 (100% over 25bp)
ATGTACACGGAGTCGAGCTCAACCCGTGGAATTCTCGGGTGCCAAGGAAC705120.2157276310161704RNA PCR Primer, Index 1 (100% over 24bp)
CGCTGAGAAGACGGTCGAACTTGACTATCTGGAATTCTCGGGTGCCAAGG702140.21481591621524548Illumina Small RNA Adapter 2 (100% over 21bp)
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAA679850.20799641188215262No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCTGGAATTCTCGGGTGC673750.20613015004133312No Hit
CTTGGTGACTCTAGATAACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAA654930.20037227334555888RNA PCR Primer, Index 1 (100% over 23bp)
AGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCAAG646190.19769831785559785No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA645650.1975331077910007No Hit
GGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCGTGGAATTCTCGGGTGC642330.1965173718382924No Hit
CTTTGGTGACTCTAGATAACCTCGGGCTGGAATTCTCGGGTGCCAAGGAA630290.19283379928690442RNA PCR Primer, Index 1 (100% over 23bp)
AGCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGC618430.1892052967570488No Hit
CCGTGATCGTATAGTGGTTAGTACTCTGCGTGGAATTCTCGGGTGCCAAG600020.18357285733254278No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC578350.17694303862917254No Hit
AGCAGCCGACTTAGAACTGGTGCGGACCAGGTGGAATTCTCGGGTGCCAA550430.16840106640037253No Hit
GCTGAGAAGACGGTCGAACTTGACTATCTTGGAATTCTCGGGTGCCAAGG548520.16781671228300118Illumina Small RNA Adapter 2 (100% over 21bp)
GGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACTC547920.1676331455445599RNA PCR Primer, Index 1 (100% over 26bp)
GGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGGA503070.15391153184607562RNA PCR Primer, Index 1 (100% over 22bp)
GGGAATACCGGGTGCTGTAGGCTTTTGGAATTCTCGGGTGCCAAGGAACT469710.1437052211887415RNA PCR Primer, Index 1 (100% over 25bp)
GGGAATACCGGGTGCTGTAGGCTTTGGAATTCTCGGGTGCCAAGGAACTC446160.13650022670492196RNA PCR Primer, Index 1 (100% over 26bp)
CGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAAGGA401800.12292852584283138RNA PCR Primer, Index 1 (100% over 22bp)
AACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAA400950.12266847296337294RNA PCR Primer, Index 6 (100% over 38bp)
AACCTCGGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCCAAT400820.12262870017004399RNA PCR Primer, Index 6 (100% over 39bp)
ATGTACACGGAGTCGAGCTCAACCTGGAATTCTCGGGTGCCAAGGAACTC379650.11615185374870816RNA PCR Primer, Index 1 (100% over 26bp)
GCTTTGGTGACTCTAGATAACCTCGGGCCGATGGAATTCTCGGGTGCCAA378470.115790839163107No Hit
GGCCGTGATCGTATAGTGGTTAGTACTCTGCTGGAATTCTCGGGTGCCAA372510.11396740956125714No Hit
AGGGGCTCTCGCTTCTGGCGCCAAGCGCCCGGCCTGGAATTCTCGGGTGC360290.11022876698833677No Hit
AGATAACCTCGGGCCGTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACG354760.10853689354903648RNA PCR Primer, Index 6 (100% over 34bp)
GTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGCC348380.10658496723027774RNA PCR Primer, Index 6 (100% over 36bp)
CTGTCCCCGCCGGCGGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCA347170.10621477430775453RNA PCR Primer, Index 1 (100% over 32bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGATGGAATTCTCGGGTGCC344370.10535812952836199No Hit
GTCTACGGCCATACCACCCTGAACGCGCCCGTGGAATTCTCGGGTGCCAA341430.10445865250999979No Hit
ATGTACACGGAGTCGAGCTCAACCCTGGAATTCTCGGGTGCCAAGGAACT339170.10376721779520438RNA PCR Primer, Index 1 (100% over 25bp)
GCAGCCGACTTAGAACTGGTGCGGACCAGGGGTGGAATTCTCGGGTGCCA329300.10074754494784563No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)