FastQCFastQC Report
Tue 4 Jul 2023
SRR5865792.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5865792.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63161402
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA27228314.3109096913333245No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA10737371.699989180100847No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA9375901.4844350668466795No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA7345201.1629254208131732No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAA6125080.9697504814728463No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA5782580.9155243260749658No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA4049170.6410829829268198No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAAA3863080.6116203690348735No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAAA3861330.6113433010875852No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA3838670.607755666981553No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAA3728010.5902354732404451No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAAA3706400.5868140799027862No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA3263830.5167443876562461No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA3101160.49098973452172573No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAA3027920.4793940451163513No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA3019520.47806411896936674No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGCAAAAAAAAAAAAAAAAAAA2646920.4190723948781251No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA2109590.33399986909726925No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAAA1924100.3046322499301076No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA1659120.2626794129743985No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1633090.2585582251641596No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA1604890.254093473099283No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAAAAAAA1597830.2529757018376508No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAAAAAAAT1563890.24760216690566814No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAA1543340.24434859758179528No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA1489330.23579748910576748No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA1259330.19938284460500103No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAAA1228990.194579278021726No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCACAAAAAAAAAAAAAAAAAA1182740.18725676798624577No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA1125930.17826235079455646No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAAAAAAT1119790.1772902381109273No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAT1062370.16819924294904032No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1057620.1674472013778288No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA1020170.1615179473058562No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA1015410.16076432248923164No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGCAAAAAAAAAAAAAAAAAA887900.14057636022708933No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGTAAAAAAAAAAAAAAAAAA828020.13109588669358543No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA801840.12695095020215036No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAAAAAAAAA800720.12677362671588574No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA775780.12282501265567221No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCAAAAAAAAAAAAAAAAAA769250.12179115340093306No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAT767370.12149350326327463No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAAAAAAAA716640.113461699282736No Hit
TGATGGTTTAGTGAGGCCCTCGGATCGGCAAAAAAAAAAAAAAAAAAAA713490.1129629769776168No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGTAAAAAAAAAAAAAAAAAA709690.11236134372064761No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTACAAAAAAAAAAAAAAAAAA708940.11224260031466686No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAAA707480.11201144648435764No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAAA693130.10973948931659243No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAAAA684890.10843489509621716No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAAA673360.1066094131349396No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA666960.10559613607057045No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA662460.10488367563468588No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAT661180.10468102022181205No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)