FastQCFastQC Report
Thu 6 Jul 2023
SRR5865793.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5865793.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40933422
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC654210.15982294370600142Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AACTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC492790.12038817570639464Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ATATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC469870.11478883930104843Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
AATTAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC466020.11384828759247151Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TAATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC427940.10454537614763798Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACATAGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTC417590.10201687999600914Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)