FastQCFastQC Report
Tue 4 Jul 2023
SRR5865794.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5865794.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53234814
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA31169815.855155237322704No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA9847011.8497312679631042No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA8028411.508112717365745No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA7661941.4392724280017208No Hit
ATGGTAATCCTGCTCAGTACGAGAGGAACCGCAAAAAAAAAAAAAAAAA6965891.3085215250305937No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAAA3958640.7436186402379465No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA3771190.7084067204592844No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA3671470.6896746178168294No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA2937780.551853153840267No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA2747610.5161302902269932No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAAA2606820.48968331137589777No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA2564220.4816810292602882No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA2379270.4469387269766736No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAA2066460.38817830752634924No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAAA2046520.3844326383858503No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAA2014180.3783576664699157No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAAA1953260.366914027350598No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA1836760.3450298520813842No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA1820480.3419717029536348No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGAAAAAAAAAAAAAAAAAA1756580.32996827978022053No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAAA1745210.3278324594127444No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAA1689920.3174463988922738No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCGAAAAAAAAAAAAAAAA1655800.31103705931986536No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAAAAAAAAAA1547630.2907176495441498No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1486610.27925522572503025No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAAAAAAAAA1428820.2683995477095121No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA1407050.2643101185626383No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTACCAAAAAAAAAAAAAAAA1355580.2546416335746003No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA1345810.2528063684039546No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAAA1256550.2360391453607784No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCGAAAAAAAAAAAAAAAAAA1146260.21532149994926256No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAAAAAAAAT1111380.2087693966583597No Hit
TCTTCCCGGAGTCGGGTTGCTTGGGAATGCAAAAAAAAAAAAAAAAAAA1034420.19431269169081722No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1011900.19008237729542926No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGAGAAAAAAAAAAAAAAAAAAA1006730.18911120831567102No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA973530.18287468798144013No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA919720.17276664101803757No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA907760.17051999092173026No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAAA905690.17013114763583095No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA885780.1663911139052726No Hit
TTCTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAAAAAAAA874860.16433982468690508No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGCAAAAAAAAAAAAAAAAAAA855440.16069183598537604No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAAA815720.15323055322406123No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTACCAAAAAAAAAAAAAAAA787530.14793514634990554No Hit
CCGGGGGTGGGGGCCGGGCCGCCCCTCCCACGGTGAAAAAAAAAAAAAA739490.13891097656507262No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA715820.13446463812196283No Hit
TCTCGCCCGTCACGTTGAACGCACGTTCAAAAAAAAAAAAAAAAAAAAT711210.1335986634610952No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAA692900.13015918492736725No Hit
GCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA690310.12967266120249804No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTACCAAAAAAAAAAAAAAAAA675050.12680611601272807No Hit
ACGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAAA658470.12369161278557299No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAAA658220.12364465103606823No Hit
GCGGCGACGACCCATTCGAACGTCTGCCCTATCGAAAAAAAAAAAAAAA635490.1193748887710963No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAT631540.11863289312892124No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCGAAAAAAAAAAAAAAAAA618090.11610635100556566No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAA604710.11359295817207138No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGCAAAAAAAAAAAAAAAAAA594020.11158487376324824No Hit
ACGGACCAAGGAGTCTAACGCGTGCGCGAGCCAAAAAAAAAAAAAAAAA568540.10679853225372404No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGGAAAAAAAAAAAAAAAAAAA562500.10566393638568926No Hit
ATGACCCGCCGGGCAGCTTCCGGGAAACCGAAAAAAAAAAAAAAAAAAA557140.1046570764763074No Hit
CTCGCCCGTCACGTTGAACGCACGTTCGAAAAAAAAAAAAAAAAAAAAT554250.10411419865203249No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAT549450.10321253306154127No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTGAAAAAAAAAAAAAAAA538460.10114809455331242No Hit
CGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAAAA533810.10027460601252407No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)