FastQCFastQC Report
Mon 10 Jul 2023
SRR5865796.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5865796.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66301023
Total Bases3.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA11295841.7037203181616067No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA6306680.9512191086402996No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA6110490.9216283133368848No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAA6051050.9126631424676509No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA5498010.8292496482294097No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA3529610.5323613181654829No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA3504220.5285318146599337No Hit
TCGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAA3202710.48305589492940404No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAAA2896190.43682433074976834No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAA2576340.38858223952291054No Hit
ACTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAAA2545350.3839081034993985No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAAA2212600.3337203409365192No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1909560.2880136555359033No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAAA1744150.26306532253657683No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGTCAAAAAAAAAAAAAAAAAA1635880.2467352577651781No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGACAAAAAAAAAAAAAAAAA1584080.23892240697402212No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA1496580.22572502388085325No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTAAAAAAAAAAAAAAAAA1309830.19755803767914712No Hit
GGGGTGGGGGCCGGGCCGCCCCTCCCACGGCAAAAAAAAAAAAAAAAAA1301120.19624433245924428No Hit
TCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA1280660.19315840722397298No Hit
AGATCCCCGAATCCGGAGTGGCGGAGATGGGCGCAAAAAAAAAAAAAAA1273330.19205284358885383No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTAAAAAAAAAAAAAAAA1254180.18916450203189172No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGGTAAAAAAAAAAAAAAA1218240.18374377119339472No Hit
TCGGCGACGACCCATTCGAACGTCTGCCCTATCAAAAAAAAAAAAAAAA1213490.18302734182547983No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTAAAAAAAAAAAAAAAA1129620.17037746159663328No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAA1089880.16438358726380436No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAAAT1076580.1623775850336427No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA1061080.16003976288570992No Hit
CGGGACCGGGGTCCGGTGCGGAGAGCCGTCCAAAAAAAAAAAAAAAAAA1043420.15737615390942006No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA1019820.15381663115514824No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGAAAAAAAAAAAAAAAAA994360.14997656974312448No Hit
CCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA913120.13772336514324973No Hit
AGTGAGGCCCTCGGATCGGCCCCGCCGGGCAAAAAAAAAAAAAAAAAAA871010.13137203026264013No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA835730.12605084539947445No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCAAAAAAAAAAAAAAAAAAA833100.12565416977050262No Hit
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAA818130.12339628605730563No Hit
GCGACGAAGCCGAGCGCACGGGGTCGGCGGCGATAAAAAAAAAAAAAAA792260.11949438547878817No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA774320.11678854487660016No Hit
ACGACGAAGCCGAGCGCACGGGGTCGGCGGCGACAAAAAAAAAAAAAAA766540.11561510898557327No Hit
TTGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAAA750450.1131882987687837No Hit
TGACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAAAA744560.11229992635256926No Hit
GTGGGGGGGCCCAAGTCCTTCTGATCGAGGCCAAAAAAAAAAAAAAAAA736950.11115213109155193No Hit
CGTGGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAA713920.10767857986142988No Hit
ACCGGGGTCCGGTGCGGAGAGCCGTTCAAAAAAAAAAAAAAAAAAATTT703800.10615220823968281No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCCAAAAAAAAAAAAAAAAA680650.1026605577413187No Hit
TGTGGGGGGCCCAAGTCCTTCTGATCGAGGCAAAAAAAAAAAAAAAAAA676870.10209043079169383No Hit
ATGGTAATCCTGCTCAGTACGAGAGGAACCGCAAAAAAAAAAAAAAAAA665460.10036949203634458No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)