FastQCFastQC Report
Tue 4 Jul 2023
SRR5882586_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5882586_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6957312
Total Bases347.8 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAAT92168413.247702560989072No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1256451.8059417200206058No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATA1173411.6865852789123157No Hit
TCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAA573000.8235939397284469No Hit
ATCGGTCGTCCGACCTGGGTATAGGGGCGCTGTAGGCACCATCAATAGAT536260.770786188688965No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAAT505850.7270767790778967No Hit
TTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAAT443150.6369557668248887No Hit
TCGGTCGTCCGACCTGGGTATAGGGGCGCTGTAGGCACCATCAATAGATC396400.5697602752327335No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACCTGTAGGCACCATCAATA339960.4886369908378408No Hit
ACCCCCGCCACGCAGTTTTATCCGGTAAAGCGCTGTAGGCACCATCAATA285040.4096984582551422No Hit
GTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGCTGTAGGCACCATCAAT276400.39727986900688084No Hit
TCCCCCCCGCCGGGTCCGCCCCCGGGGCCGCGGTCTGTAGGCACCATCAA245440.35277992420061083No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCGCTCCTGTAGGCACC237000.3406488022960592No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTTCTGTAGGCACCATC231910.3333327583986459No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCAAT194970.28023754001545426No Hit
ATAAGGCCTACTTCACAAAGCGCCTTCCCCCGCTGTAGGCACCATCAATA183570.2638519014239982No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACCTGTAGGCACCATCAATA169530.24367169389557347No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTTCTGTAGGCACCATCAATAGA159430.22915459303823082No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAG149670.21512618666519484No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACACTGTAGGCACCATCA147940.21263959414210545No Hit
TCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTGTAGGCACCATCAATAG146670.21081417650954853No Hit
ACTGGTGCGGACCAGGGGAATCCGACTGTCTGTAGGCACCATCAATAGAT142840.2053091768775067No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGCTGTAGGCACCATCAATAGA141240.20300943812782868No Hit
CCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAATAG137130.1971019842145932No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTCTGTAGGCACCATCAATA132050.18980031368436545No Hit
TGCCCTCGGGCCCTCACGGAGGCGGGGGTTCCAGGGCACTGTAGGCACCA129410.1860057447473967No Hit
TCGAGGCCAGCCTGGTCCACATGGGTCGGCTGTAGGCACCATCAATAGAT123720.17782729881885417No Hit
TTCTCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATAGAT122930.17669180281120064No Hit
AGCGGCGGGGGCGGCGCGCGCGCGCGCGCGCTGTAGGCACCATCAATAGA122630.17626060179563602No Hit
TCCCCCCCGCCGGGTCCGCCCCCGGGGCCGCGGCTGTAGGCACCATCAAT121840.1751251057879825No Hit
TGGGCGAGCGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAGA121080.17403272988188542No Hit
TTTCCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCCTGTAGGCACCATCAA112610.1618584878757773No Hit
GCTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTCCCTGTAGGCACCATCA108370.15576418018913052No Hit
TGCCCTCGGGCCCTCACGGAGGCGGGGGTTCCAGGGCACCTGTAGGCACC107300.15422622990028334No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCCTGTAGGCACCATCAATA101360.1456884497921036No Hit
AACTGGTGCGGACCAGGGGAATCCGACTGTTCTGTAGGCACCATCAATAG99810.143460577878353No Hit
TGCCCTCGGGCCCTCACGGAGGCGGGGGTTCCAGGGCACGCTGTAGGCAC99770.14340308440961108No Hit
TTCGAGGCCAGCCTGGTCCACATGGGTCGGCTGTAGGCACCATCAATAGA98860.14209510799573166No Hit
ATGGGCGAGCGCCGTTCCGAAGGGACGGGCGCTGTAGGCACCATCAATAG93030.13371543492659232No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATAGA86960.12499080104500129No Hit
TTCCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAA86030.12365407789675094No Hit
ACCTCACCCGGCCCGGACACGGACAGGCTGTAGGCACCATCAATAGATCG85440.12280604923280715No Hit
AGGTTCTCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATA84920.12205863413916179No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCGCTGTAGGCACCATCAATA84400.12131121904551644No Hit
ACGGAAGGGCACCACCAGGAGTGGAGCCTGCGGCTCTGTAGGCACCATCA82880.11912646723332229No Hit
ACCGCCTGGGAATACCGGGTGCTGTAGGCTTCTGTAGGCACCATCAATAG79620.1144407495308533No Hit
AATCGGTCGTCCGACCTGGGTATAGGGGCGCTGTAGGCACCATCAATAGA78860.11334837362475622No Hit
TCCGCGCGGCGCCTCGCCTCGGCCGGCGCCTAGCCTGTAGGCACCATCAA77720.11170980976561062No Hit
TGTCGGCGGCCCGGCGGATCTTTCCCGCCCCCCGCTGTAGGCACCATCAA76260.10961129815652941No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACCTGTAGGCACCATCAA75000.10780025389115797No Hit
TCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGCTGTAGGCACCATCAAT74480.10705283879751261No Hit
AGCATCGCGAAGGCCCGCGGCGGGTGTTGACGCGATGTGCTGTAGGCACC72350.10399131158700373No Hit
TCCCTACCTACTATCCAGCGAAACCACAGCCCTGTAGGCACCATCAATAG70660.10156221253265628No Hit
TCTCTCGGGGCCACGCGCGCGTCCCCCGCTGTAGGCACCATCAATAGATC69950.10054170346248666No Hit
TCGGTCGTCCGACCTGGGTATAGGGGCGACTGTAGGCACCATCAATAGAT69760.10026860948596239No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)