FastQCFastQC Report
Tue 4 Jul 2023
SRR5886312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81676459
Total Bases6.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG6503250.796220854775303No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2629900.3219899628606573No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1144810.14016400980360816Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GCAACATAGCGAGACCCCGTCTCTACTGTAGGCACCATCAATAGATCGGA1135120.13897762144659087No Hit
AACATAGCGAGACCCCGTCTCTACTGTAGGCACCATCAATAGATCGGAAG899220.11009537031961682No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)