FastQCFastQC Report
Tue 4 Jul 2023
SRR5886313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75982588
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG19255542.5342042837498506No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA10442541.3743332880422552No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC2671390.35157923286319226No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA1893740.2492334164769434Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA1870630.24619193018274133No Hit
GCAACATAGCGAGACCCCGTCTCTACTGTAGGCACCATCAATAGATCGGA1817810.2392403375362787No Hit
AACATAGCGAGACCCCGTCTCTACTGTAGGCACCATCAATAGATCGGAAG1213060.15964973448917008No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAG1206280.15875742479316446No Hit
TCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA1151960.15160841849714304No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAG842410.11086882168319931No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)