FastQCFastQC Report
Tue 4 Jul 2023
SRR5886314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74935693
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG40649235.424548485859735No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA16341682.180760508880595Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA15935852.1266034064701316No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC3522510.47007105145474537No Hit
ACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA2546740.3398567355612498No Hit
TACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAG2423580.3234213100558101No Hit
TGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGA2253850.30077122260015665No Hit
ACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGC1540770.20561229746684268No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAG1537180.2051332200264032No Hit
TCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1280420.17086917445335428No Hit
TCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGA1150020.15346758720173576No Hit
AACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1080890.14424234389878798No Hit
GTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGAAG933180.12453077600817009No Hit
GAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA907680.12112785825574471No Hit
TGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAAT812000.1083595770576246No Hit
ATGTACACGGAGTCGACCCAACGCCTGTAGGCACCATCAATAGATCGGAA810950.10821945691487767No Hit
GCAACATAGCGAGACCCCGTCTCTACTGTAGGCACCATCAATAGATCGGA801100.10690499652815648No Hit
GAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGGAAGAGCACA764680.10204482929116303No Hit
TAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATCGGA750880.10020325027220338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)