FastQCFastQC Report
Tue 4 Jul 2023
SRR5886315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84636027
Total Bases6.4 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA7493380.8853652830372106Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG7146970.8444359043460298No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA3897990.46055918952811903No Hit
GCAACATAGCGAGACCCCGTCTCTACTGTAGGCACCATCAATAGATCGGA1376320.16261632885957655No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAATAGATC1083390.12800577229363566No Hit
AGTGGGGGACTGCGTTCGCGCTTTCCCCTGCTGTAGGCACCATCAATAGA934980.11047068643711265No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)