FastQCFastQC Report
Tue 4 Jul 2023
SRR5886316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74275875
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA40434725.443856433869005No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC38969295.246560878616374No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC23547763.1703106829774805No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA15475532.0835203893592635No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA11709671.576510542622352No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA9292351.2510589743978109No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG7565641.0185864521959518No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC7346550.9890896606738055No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG4452290.5994261259123503No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA4034100.5431238608767651No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA3368440.4535039136193279No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG3345860.4504638955784768No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG3245390.436937296262077No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA3230560.4349406856533161No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA3062730.41234519283683974No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT3052490.41096654869431026No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG2275300.30633095873996774No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC2075630.2794487442928138No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA2050370.2760479092302851No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1954220.26310292541151487No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCTGTAGGCACCATCAAT1900750.255904087296178No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1859200.25031007712800424No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA1750970.23573872404734378No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA1723760.23207535421157946No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1579050.21259258137315784No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1565870.21081811557251934No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1514690.20392758752421294No Hit
TCGTTTCCCGGTCAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1421420.19137034737052913No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1197120.1611721167875841No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1182940.15926301776990173No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAG1151620.15504630541208703No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1131060.1522782464696646No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1118520.1505899459279342No Hit
GTCTAGGGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1073120.14447759787414152No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA1070740.14415717081757703No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1059070.14258600117467482No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT1051690.1415924080328909No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT1024760.13796673549789887No Hit
GTCTAGCGGTTAGGATTCCTGGTTCTGTAGGCACCATCAATAGATCGGAA1014350.13656520370847197No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCCTGTAGGCACCATCAA1010000.13597954921433103No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCCTGTAGGCACCATCA976200.1314289464782475No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG963010.12965313434543854No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGC959240.12914556711718309No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTGTAGGCACCATC918000.12359329324629295No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG915510.12325805653585367No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAACGCCTGTAGGCACCATCAA906220.1220073139495159No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA854620.11506023995005107No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAACGCTGTAGGCACCATCAAT840660.11318076024011835No Hit
TAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC837880.11280647989673633No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG830970.11187616436696302No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA830590.11182500374448635No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA829190.1116365172406249No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGACTGTAGGCACCATCA823630.11088795655386087No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG819010.11026595109111807No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG803850.10822491152073267No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGA797360.10735114194211783No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG774260.10424111462840392No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT764010.10286112415370402No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG761360.1025043461285377No Hit
GTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC757320.10196042793168038No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATAGATCGG755690.10174097578789883No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA747190.10059659344302575No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA743660.10012133818686081No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)