FastQCFastQC Report
Tue 4 Jul 2023
SRR5886317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73508643
Total Bases5.5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA40330235.486460959427587No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC38612305.252756468378827No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC23429863.1873612467584254No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA15361982.089819560401897No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA11602901.5784402386532967No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA9235161.2563366188109335No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG7582351.0314909499825755No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC7272170.9892945513903718No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG4470450.6081529759704584No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA4058000.552043927678001No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG3400290.46257009532879007No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA3334850.4536677408124647No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG3283620.44669849231198566No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA3239070.4406379804889066No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA3051170.4150763604764136No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT3029380.4121120832008829No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG2256990.30703736430014084No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA2148490.2922771952136295No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC2073030.2820117356812042No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1966590.2675318057496994No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCTGTAGGCACCATCAAT1903940.2590089984384557No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1869340.2543020689417434No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA1753400.23852977397501407No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA1716020.23344465765746755No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1689440.2298287563273342No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1514820.2060737265956603No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1489150.20258162023205897No Hit
TCGTTTCCCGGTCAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1409830.19179105238005822No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1200500.16331412892494832No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1182400.16085183343678378No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAG1154080.15699922524756715No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1137750.15477771777122862No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1110200.15102985916907757No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA1070020.14556383526220176No Hit
GTCTAGGGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1062930.14459932283065No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT1060990.14433540828661467No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1054930.14351101543256622No Hit
GTCTAGCGGTTAGGATTCCTGGTTCTGTAGGCACCATCAATAGATCGGAA1028770.13995225024083222No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT1020130.13877687825090174No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCCTGTAGGCACCATCAA1009340.13730902364773623No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCCTGTAGGCACCATCA976170.13279662909843135No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGC963980.13113832070060114No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG957520.1302595124766485No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG922220.12545735608260378No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTGTAGGCACCATC912270.12410377375623707No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAACGCCTGTAGGCACCATCAA900400.12248899765433025No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG860620.11707738911735863No Hit
TCCTTAGGTCGCTGGTTCGTTTCCGGCTCGAAGGACTGTAGGCACCATCA851280.1158067902300958No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA850550.11570748218002065No Hit
TAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC839560.11421241989190306No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAACGCTGTAGGCACCATCAAT836590.11380838577036445No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG833330.11336490050564531No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA821870.11180590015789028No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA821650.11177597170444298No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG813070.11060876201999811No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG787170.10708536681870186No Hit
AGCAGAGTGGCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGA785910.10691395840350365No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT763910.10392111305877325No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATAGATCGG762950.10379051617100318No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG757570.10305862944579183No Hit
GTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC753220.10246686229808377No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA747390.10167375828173023No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA741870.10092282617705241No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)