FastQCFastQC Report
Mon 10 Jul 2023
SRR5886318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78424335
Total Bases5.9 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC52944986.751090716931167No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA23613983.0110526279884935No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC21580362.7517428104427024No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA11131031.4193336800369427No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG10262661.3086065696317348No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA9616951.2262711567780586No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA6394310.8153476851286021No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG5015870.6395808137869451No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA4236750.5402340995304582No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG4035460.5145673214825475No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA3822200.48737423148057296No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC3667780.46768391469306053No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG3654670.4660122396957526No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA3474880.44308695763885025No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG3452120.4401847972316246No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA3308790.4219085823297067No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC3073380.3918911138997863No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT2872760.36630976851764185No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG2264390.28873563288741944No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA1993800.25423231194756063No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1975520.2519014027979963No Hit
TCGTTTCCCGGTCAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCAC1919460.2447531113907437No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1797700.22922731828073517No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA1645360.20980222529142262No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA1614700.20589272449680318No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT1589390.20266540991389978No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1526890.1946959448237591No Hit
GTCTAGGGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1501600.1914711804696846No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG1411870.18002957882907136No Hit
GCGCAGCGGAAGCGTGCTGGCTGTAGGCACCATCAATAGATCGGAAGAGC1408500.17959986527141097No Hit
GTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1400880.17862822808762102No Hit
GTCTAGCGGTTAGGATTCCTGGTTCTGTAGGCACCATCAATAGATCGGAA1398900.17837575543356535No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1387570.17693105080202465No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATAGATCGG1316770.16790324074791327No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCTGTAGGCACCATCAAT1253410.15982411581813222No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1241300.15827995226226657No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAG1229430.15676639145234703No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1218190.15533316285053614No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA1198080.15276890776313246No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1186680.15131527733069078No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1186680.15131527733069078No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1168050.14893973917662165No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATAGATCG1129960.1440828283720863No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTGTAGGCACCATC1123960.14331775972343277No Hit
GTCTAGGGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC1097620.13995910835584388No Hit
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATAGATCGGAAGA1062520.13548345676122087No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1061500.13535339509094976No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG1059520.13510092243689412No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1025790.13079996151704698No Hit
ATCCCAGCGGTGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT1024200.1305972183251538No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG1007440.12846012656658165No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1004510.12808651804315588No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCCTGTAGGCACCATCA946470.1206857539818476No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC924810.11792385616020842No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT918190.11707973041786074No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA915580.11674692555569645No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA901740.1149821672061357No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA896480.11431145702414947No Hit
GTATAGTGGTGAGTATCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA895330.11416481886649087No Hit
AGCAGTTGACCCGGGTTCGATTCCCGGCCAACGCTGTAGGCACCATCAAT889780.11345713036648637No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA855560.10909368884033253No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA839170.10700377631509403No Hit
GGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATAGATCGGAA838680.10694129570878733No Hit
GTCTAGGGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA835240.10650265635022597No Hit
TTCTTGCGACCCGGGTTCGTTTCCCGGGCGGCGCTGTAGGCACCATCAAT792860.10109872146190338No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)