FastQCFastQC Report
Fri 7 Jul 2023
SRR5886321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89601931
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC47845325.339764385211742No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA33666023.7572873289974074No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC23962592.6743385697792608No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG11580531.2924420122151161No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC9526301.0631802120425284No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA8959240.9998936295245691No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA6715930.7495296055617373No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA6034120.673436379401243No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA5209070.5813568906232612No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG4900360.5469033920708696No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA4830960.5391580232796545No Hit
TCCCCGGCACCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4710390.5257018400641388No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG4415280.49276616594345496No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA3671730.4097824632819576No Hit
TCCCCGGCATCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3594680.40118331824790693No Hit
TCGTTTCCCGGTCAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCAC3547230.39588767344757336No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG3434930.3833544614122211No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT3281620.366244339086844No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA3175240.35437182709823517No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC2964680.3308723335437938No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG2959090.33024846306046685No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG2919550.32583561173475156No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG2917970.32565927624930313No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA2899370.3235834281294674No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA2581620.2881210227489405No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG2537980.2832505919989604No Hit
TCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGATC2189080.24431169904139677No Hit
GTCTAGGGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG2081240.2322762441358546No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA2004710.22373513356536925No Hit
TCCCCGACACCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1908410.21298759733202624No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1758400.19624577064081355No Hit
TGCCCGCATCCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1711070.190963518408995No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA1590740.17753412033050942No Hit
AACCGGGCGGAAACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1506640.1681481618961984No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCTGTAGGCACCATCAATAGAT1442910.16103559196732042No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1436850.16035926725730945No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCTGTAGGCACCATCAATAGATCGG1417590.15820976001064083No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1411550.15753566739538236No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1411310.15750888225835222No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG1380110.15402681444443422No Hit
GTATAGTGGTGAGTATCCCCCTGTAGGCACCATCAATAGATCGGAAGAGC1367200.15258599728168806No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1354180.15113290359780304No Hit
GTGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACCCTGTAGGCACCATC1341450.14971217528782946No Hit
GCGCAGCGGAAGCGTGCTGGCTGTAGGCACCATCAATAGATCGGAAGAGC1331470.1485983600063262No Hit
GTCTAGGGGTATGATTCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC1319420.147253522917938No Hit
ATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGATC1318600.14716200703308505No Hit
ATCCCACTTCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1302950.14541539288924477No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA1219140.1360617998288452No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG1200650.1339982282301483No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1168200.13037665449419836No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1161060.12957979666755173No Hit
GTCTAGGGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1155900.12900391622140375No Hit
GTCTAGCGGTTAGGATTCCTGGCTGTAGGCACCATCAATAGATCGGAAGA1131640.12629638528660728No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGAT1130830.12620598544913056No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCCTGTAGGCACCATCAATAGATCG1121010.12511002692564738No Hit
GTATAGTGGTGAGTATCCCCTGTAGGCACCATCAATAGATCGGAAGAGCA1112030.12410781638176971No Hit
GTCTAGGGGTATGATTCTCGGTCTGTAGGCACCATCAATAGATCGGAAGA1103540.12316029215932857No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1066670.11904542548307358No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGGCGCTGTAGGCACCATCAATAG1058570.1181414271083064No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAG1041490.11623521818966155No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1013310.11309019668337282No Hit
TTGGTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGG1006150.1122911067619737No Hit
TCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA995660.1111203730642814No Hit
CCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATCG992030.11071524786670055No Hit
TTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGAT960020.10714278021530586No Hit
GTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC957740.10688832141351953No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCGCTGTAGGCACCATCAATAGATCG949640.10598432303875237No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC922610.10296764698073305No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTGTAGGCACCATC916420.10227681365483073No Hit
GTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATAGATCGGAAGA909040.10145317069115396No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA898760.10030587398836303No Hit
TCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG898480.10027462466182789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)