FastQCFastQC Report
Tue 4 Jul 2023
SRR5886322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89926731
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC44806824.982591883608No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA26279592.9223335161599504No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC23363252.598031724293414No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA13063791.4527148774039167No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA8672970.9644484908497342No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG8394630.9334966262701132No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA7203860.8010810489708561No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG7038200.7826593852277361No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA5466280.6078593027027748No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG5145080.5721413358170442No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG4886960.5434379684056346No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG4762300.5295755719175425No Hit
TCCCCGGCACCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4547130.5056483149598755No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA4544660.5053736469081702No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC4430310.49265773933225704No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG3764410.4186085670121824No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA3468210.38567064113561517No Hit
TCCCCGGCATCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3225500.3586808910022538No Hit
TCGTTTCCCGGTCAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCAC2802390.3116303649467698No Hit
TGCCCGCATCCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2554550.28407015039832817No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA2547860.28332621142427605No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAG2294350.25513548357495613No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT2256270.25090092510979856No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA2233040.24831771100408398No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG2167090.2409839628219111No Hit
TCCCCGACACCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2114310.23511474024336546No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2113260.2349979785209806No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1957980.2177305878048653No Hit
AACCGGGCGGAAACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1804110.20061999140166675No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA1765440.19631982396869288No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA1742810.19380333084719825No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA1728790.19224428384925946No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1723070.1916082104663629No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1693750.18834777837081612No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1613560.1794305188298238No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1510480.16796785374084153No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTGTAGGCACCATC1500670.16687696564884583No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1490470.16574270891710718No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1463800.16277696116853174No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1364920.15178134296908893No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT1347670.14986311467276622No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG1341790.149209249027411No Hit
GTCTAGGGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1331620.14807832834488335No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG1322920.14711087407369453No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1285600.14296082885521547No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTCTGTAGGCACCATCAATA1269890.14121385108505724No Hit
GCGCAGCGGAAGCGTGCTGGCTGTAGGCACCATCAATAGATCGGAAGAGC1259540.1400629141072636No Hit
GCCTGGGTAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1221140.13579277111718874No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA1195590.1329515692058238No Hit
GTCTAGGGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1155550.12849905552554777No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1139190.1266797966891513No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1133010.12599257055168614No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA1121670.12473154394992965No Hit
ATCCCACTTCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1120810.12463591053921443No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCTGTAGGCACCATCAAT1092020.12143441531306191No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1052970.11709199125674878No Hit
CCCCCCGGCTCCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCA1041480.1158142844089373No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1008280.1121223899487684No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA1004370.11168759153493525No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC994890.11063339998426051No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACCACTGTAGGCACC976330.10856949753905766No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA950900.1057416398245367No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT924920.10285262120781416No Hit
GTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC920680.10238112625266006No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG912110.10142812819471889No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA903290.10044732972668605No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)