FastQCFastQC Report
Fri 7 Jul 2023
SRR5886323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5886323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88773976
Total Bases6.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC44214614.980582372473663No Hit
TCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA26069452.936609485644757No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACC23123632.6047757509475526No Hit
AGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCA12925971.4560539678880666No Hit
GTGTAATGGTTAGCACTCTGGACTCTGTAGGCACCATCAATAGATCGGAA8610160.9698968535553708No Hit
GCCCGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG8414340.9478385872904915No Hit
CCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA7100870.7998819383734711No Hit
GTCTAGGGGTATGATTCTCGCTTCTGTAGGCACCATCAATAGATCGGAAG7002320.7887807120411054No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA5462930.6153751635501828No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGGGACTGTAGGCACCATCAATAG5192160.5848741077002115No Hit
GCCCGGCTAGCTCAGTCGGTAGAGCATGAGACTGTAGGCACCATCAATAG4934200.5558160423050107No Hit
ATGTACACGGAGTCGACCCAACGCGACTGTAGGCACCATCAATAGATCGG4732970.5331483632095063No Hit
GTGTAATGGTTAGCACTCTGGACTGTAGGCACCATCAATAGATCGGAAGA4525840.5098160749271836No Hit
TCCCCGGCACCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC4521860.5093677453401434No Hit
TCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCAC4369530.4922084373014902No Hit
GCGCAGCGGAAGCGTGCTGGGCTGTAGGCACCATCAATAGATCGGAAGAG3726250.4197457597258007No Hit
GCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGA3614040.40710579415751297No Hit
TCCCCGGCATCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC3234390.36433988267011946No Hit
TCGTTTCCCGGTCAGGGACTGTAGGCACCATCAATAGATCGGAAGAGCAC2796730.3150393984831771No Hit
TGCCCGCATCCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2568190.2892953673720776No Hit
GCGCAGCGGAAGCGTGCTGGGCCTGTAGGCACCATCAATAGATCGGAAGA2517950.28363605117788127No Hit
GTTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAG2286090.25751803659216527No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGCTGTAGGCACCATCAAT2251370.2536069804961761No Hit
GCATGGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA2243980.2527745293282797No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAG2158680.2431658575256334No Hit
TCCCCGACACCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC2116690.23843586773673403No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGACTGTAGGCACCATCAA2111810.23788615708729774No Hit
GCCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA1943540.21893127778798596No Hit
AACCGGGCGGAAACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1808030.20366666915988982No Hit
GCGCCGCTGGTGTAGTGGTATCATGCAAGATTCTGTAGGCACCATCAATA1762340.19851989055891783No Hit
TCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGCTGTAGGCACCATCAATA1760740.19833965755910268No Hit
GTATAGTGGTGAGTATCCCCGCCTGTAGGCACCATCAATAGATCGGAAGA1719040.19364233500141978No Hit
CCTCCCGGGGCTACGCCTGTCTGAGCGTCGCTCTGTAGGCACCATCAATA1701070.19161809312224565No Hit
TGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATC1680840.18933927213083257No Hit
GCCTGGATAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1601190.1803670481087836No Hit
ATCCTGCCGACTACGCCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1496790.16860684487084368No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTGTAGGCACCATC1493190.16820132062125953No Hit
GCATTGGTGGTTCAGTGGTAGAATTCTCGCCTGTAGGCACCATCAATAGA1487670.1675795167718972No Hit
TCCCTGGTGGTCTAGTGGTTAGGATTCGCTGTAGGCACCATCAATAGATC1478660.16656457969168803No Hit
TGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1457330.16416184851290203No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCCTGTAGGCACCAT1349150.15197584481289877No Hit
GTCTAGCGGTTAGGATTCCTGGTCTGTAGGCACCATCAATAGATCGGAAG1343510.1513405234885503No Hit
GTATAGTGGTGAGTATCCCCGCTGTAGGCACCATCAATAGATCGGAAGAG1323280.14906170249713724No Hit
GTCTAGGGGTATGATTCTCGCCTGTAGGCACCATCAATAGATCGGAAGAG1316760.1483272530228904No Hit
GCCGTGATCGTATAGTGGTTAGTACTCTGCGTCTGTAGGCACCATCAATA1302290.14669727083081194No Hit
TTTCCGTAGTGTAGTGGTTATCACGTTCGCCTGTAGGCACCATCAATAGA1286290.14489494083266022No Hit
GCGCAGCGGAAGCGTGCTGGCTGTAGGCACCATCAATAGATCGGAAGAGC1251540.1409805053679245No Hit
GCCTGGGTAGCTCAGTCGGTAGAGCATCAGACTGTAGGCACCATCAATAG1221390.13758423977765735No Hit
TCGCCGGCCGAGGTGGGATCCCGAGGCCTCCTGTAGGCACCATCAATAGA1183960.1333679140382312No Hit
GTCTAGGGGTATGATTCTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1148050.12932280964862947No Hit
GCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCACCATCA1131970.127511468000487No Hit
AGGGGGGTCTCCCCCGCGGGGGCGCGCCGGCCTGTAGGCACCATCAATAG1131720.12748330659426585No Hit
ATCCCACTTCTGACACCACTGTAGGCACCATCAATAGATCGGAAGAGCAC1118260.12596709648332075No Hit
CCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGACTGTAGGCA1105570.12453762350353667No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCTGTAGGCACCATCAAT1081270.12180033481884375No Hit
CGCGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAG1049000.11816526050382153No Hit
CCCCCCGGCTCCTCCACCACTGTAGGCACCATCAATAGATCGGAAGAGCA1040180.1171717260923404No Hit
CCGCCGCGGCCCGGGTTCGATTCCCGGTCAGGGACTGTAGGCACCATCAA1002400.11291597438420468No Hit
TGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCCTGTAGGCACCATC998860.1125172088721136No Hit
ACCAGTGCGCCCCGGGCGGGTCGCGCCGTCGGGCCCGGGGGCTGTAGGCA981020.11050761092417445No Hit
TGCACGAGGCCCCGGGTTCAATCCCCGGCACCTCCACCACTGTAGGCACC974510.10977428790617647No Hit
CGCGCGGGTCGGGGGGCGGGGCGGACTGTCTGTAGGCACCATCAATAGAT967800.1090184357632016No Hit
CTCGCCGGCCGAGGTGGGATCCCGAGGCCTCTGTAGGCACCATCAATAGA944560.10640055144088623No Hit
GTCTAGGGGTATGATTCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC914530.10301780332560526No Hit
GTGTAATGGTTAGCACTCTGGCTGTAGGCACCATCAATAGATCGGAAGAG911970.10272943052590097No Hit
AGCAGTTGACCCGGGTTCGTTTCCCGGCCAACGCCTGTAGGCACCATCAA897230.1010690340151037No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)